Comparison of PCR, electrochemical enzyme-linked immunosorbent assays, and the standard culture method for detecting Salmonella in meat products

被引：59

作者：

Croci, L

Delibato, E

Volpe, G

De Medici, D

Palleschi, G

机构：

[1] Ist Super Sanita, Lab Alimenti, I-00161 Rome, Italy

[2] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 03期

关键词：

D O I：

10.1128/AEM.70.3.1393-1396.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with flow injection analysis (ELISA-FIA) and a PCR-based method using ST11 and ST15 primers for detecting salmonellae in meat were evaluated in comparison with the International Organization for Standardization (ISO) culture method. The methods were applied to experimentally contaminated and naturally contaminated meat samples. The results showed that both ELISA-FIA and PCR allowed detection of salmonella in a product contaminated with a low number of the microorganisms (1 to 10 salmonellae/25 g) after only 5 h of incubation of preenrichment broth, and they were just as effective as the ISO method.

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页码：1393 / 1396

页数：4

相关论文

共 26 条

[1] SALMONELLA IDENTIFICATION BY THE POLYMERASE CHAIN-REACTION [J].

AABO, S ;

RASMUSSEN, OF ;

ROSSEN, L ;

SORENSEN, PD ;

OLSEN, JE .

MOLECULAR AND CELLULAR PROBES, 1993, 7 (03) :171-178

[2] Piezoelectric flow injection analysis biosensor for the detection of Salmonella typhimurium [J].

Babacan, S ;

Pivarnik, P ;

Letcher, S ;

Rand, A .

JOURNAL OF FOOD SCIENCE, 2002, 67 (01) :314-320

[3] SEROLOGICAL DIAGNOSIS OF SALMONELLA SEROTYPE ENTERITIDIS INFECTIONS IN POULTRY BY ELISA AND OTHER TESTS [J].

BARROW, PA .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 21 (1-2) :55-68

[4] Epidemiology -: Tracing the origins of Salmonella outbreaks [J].

Bäumler, AJ ;

Hargis, BM ;

Tsolis, RM .

SCIENCE, 2000, 287 (5450) :50-52

[5] EXPERIMENTAL ENZYME-LINKED AMPEROMETRIC IMMUNOSENSORS FOR THE DETECTION OF SALMONELLAS IN FOODS [J].

BROOKS, JL ;

MIRHABIBOLLAHI, B ;

KROLL, RG .

JOURNAL OF APPLIED BACTERIOLOGY, 1992, 73 (03) :189-196

[6] A rapid electrochemical ELISA for the detection of salmonella in meat samples [J].

Croci, L ;

Delibato, E ;

Volpe, G ;

Palleschi, G .

ANALYTICAL LETTERS, 2001, 34 (15) :2597-2607

[7] Enzyme-linked immunomagnetic electrochemical detection of Salmonella typhimurium [J].

Gehring, AG ;

Crawford, CG ;

Mazenko, RS ;

VanHouten, LJ ;

Brewster, JD .

JOURNAL OF IMMUNOLOGICAL METHODS, 1996, 195 (1-2) :15-25

[8] RAPID DETECTION OF SALMONELLA-ENTERITIDIS IN POOLED LIQUID EGG SAMPLES USING A MAGNETIC BEAD-ELISA SYSTEM [J].

HOLT, PS ;

GAST, RK ;

GREENE, CR .

JOURNAL OF FOOD PROTECTION, 1995, 58 (09) :967-972

[9] Automated 5′ nuclease PCR assay for identification of Salmonella enterica [J].

Hoorfar, J ;

Ahrens, P ;

Rådström, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) :3429-3435

[10] An overview of the Salmonella Enteritidis risk assessment for shell eggs and egg products [J].

Hope, BK ;

Baker, AR ;

Edel, ED ;

Hogue, AT ;

Schlosser, WD ;

Whiting, R ;

McDowell, RM ;

Morales, RA .

RISK ANALYSIS, 2002, 22 (02) :203-218