A photometric approach for detection of interaction of cells with their ligands based on lectin conjugated gold nanoparticles

被引：6

作者：

Mikaelyan, Mariam V. ^{[1
]}

Poghosyan, Gayane G. ^{[1
]}

Gasparyan, Vardan K. ^{[1
]}

机构：

[1] Natl Acad Sci, Inst Biochem, Yerevan 0014, Armenia

来源：

ANALYTICAL LETTERS | 2008年 / 41卷 / 07期

关键词：

cell interactions; gold nanoparticles; lectins;

D O I：

10.1080/00032710802052577

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple photometric approach for detection of interaction of lectins immobilized on colloidal gold particles with lymphocytes was demonstrated. It was shown that 20 nm gold particles conjugated with germ agglutinin or Concanavalin A undergo drastic optical changes upon interaction with lymphocytes. As a result of binding with cells, agglutination of gold-lectin-lymphocyte complex occurs with decrease of absorbance at 520 nm. This approach permitted to detect the interaction of 3000 lymphocytes/ml in 1.5h without any additional procedures.

引用

页码：1200 / 1207

页数：8

共 27 条

[1] PURIFICATION, COMPOSITION AND SPECIFICITY OF WHEAT-GERM AGGLUTININ [J].

ALLEN, AK ;

NEUBERGER, A ;

SHARON, N .

BIOCHEMICAL JOURNAL, 1973, 131 (01) :155-162

[2] A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus [J].

Brüning, A ;

Bellamy, K ;

Talbot, D ;

Anderson, J .

JOURNAL OF VIROLOGICAL METHODS, 1999, 81 (1-2) :143-154

[3] Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples [J].

Domínguez, J ;

Galí, N ;

Matas, L ;

Pedroso, P ;

Hernández, A ;

Padilla, E ;

Ausina, V .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1999, 18 (12) :896-898

[4]

Englebienne P, 2000, CLIN CHEM, V46, P2000

[5]

FAULK WP, 1971, IMMUNOCHEMISTRY, V8, P1081

[6] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].

FRENS, G .

NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22

[7] PROTEIN-CARBOHYDRATE INTERACTION .I. INTERACTION OF POLYSACCHARIDES WITH CONCANAVALIN A [J].

GOLDSTEIN, IJ ;

HOLLERMAN, CE ;

MERRICK, JM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 97 (01) :68-+

[8] Using biofunctionalized nanoparticles to probe pathogenic bacteria [J].

Ho, KC ;

Tsai, PJ ;

Lin, YS ;

Chen, YC .

ANALYTICAL CHEMISTRY, 2004, 76 (24) :7162-7168

[9] COLLOIDAL GOLD, A USEFUL MARKER FOR TRANSMISSION AND SCANNING ELECTRON-MICROSCOPY [J].

HORISBERGER, M ;

ROSSET, J .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (04) :295-305

[10]

Jayasena SD, 1999, CLIN CHEM, V45, P1628

← 1 2 3 →