Plasmodium falciparum glycosylphosphatidylinositol toxin interacts with the membrane of non-parasitized red blood cells:: a putative mechanism contributing to malaria anemia

被引：25

作者：

Brattig, Norbert W. ^{[1
]}

Kowalsky, Katharina ^{[1
]}

Liu, Xinyu ^{[2
]}

Burchard, Gerd D. ^{[3
]}

Kamena, Faustin ^{[2
]}

Seeberger, Peter H. ^{[2
]}

机构：

[1] Bernhard Nocht Inst Trop Med, Sect Trop Med, D-20359 Hamburg, Germany

[2] Swiss Fed Inst Technol, Organ Chem Lab, Zurich, Switzerland

[3] Univ Med Ctr, Bernhard Nocht Inst Trop Med, Hamburg, Germany

来源：

MICROBES AND INFECTION | 2008年 / 10卷 / 08期

基金：

瑞士国家科学基金会;

关键词：

Plasmodium falciparum; glycosylphosphatidylinositol; anemia; red blood cell; membrane interaction;

D O I：

10.1016/j.micinf.2008.05.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Following exposure to synthetic Plasmodium falciparum glycosylphosphatidylinositol (P.f.-GPI), red blood cells (RBCs) reacted with antibodies in the serum of a patient with severe acute P. falciparum malaria. Carbohydrate microarray analysis of the patient's serum confirmed the presence of both, IgM and IgG antibodies against P.f.-GPI. The antibodies failed to bind to RBCs when P.f.-GPI lacking the lipid portion was applied. Addition of the detergent Triton X-100 during preincubation with P.f.-GPI resulted in increased recognition. Recognition of P.f.-GPI was dependent on the concentrations of synthetic P.f.-GPI, the serum and the numbers of RBCs. IgM antibodies dominated P.f.-GPI-sensitized RBCs recognition. Recognition by IgM antibodies proved highest during the 1st week of acute malaria and decreased during the following 2 weeks as assessed by flow cytometry and carbohydrate microarray analysis. These results strongly support the notion that released P.f.-GPI can insert into non-parasitized RBC membranes and results in recognition by circulating anti-GPI antibodies and possibly subsequent elimination. This process may contribute to malaria-associated anemia. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：885 / 891

页数：7

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