Conjugated-polymer/DNA interpolyelectrolyte complexes for accurate DNA concentration determination

被引：85

作者：

Hong, JW

Henme, WL

Keller, GE

Rinke, MT

Bazan, GC ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Dept Mat, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[3] Univ Munster, Inst Phys Chem, D-48149 Munster, Germany

来源：

ADVANCED MATERIALS | 2006年 / 18卷 / 07期

关键词：

D O I：

10.1002/adma.200501605

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Precise determination of DNA concentration without the health risks of intercalating dyes is possible via the optical changes that arise from DNA-induced aggregation of cationic conjugated polyelectrolytes. Addition of DNA to a conjugated electrolyte containing two fluorophores results in aggregation and fluorescence quenching, inducing a measurable and quantifiable shift from blue- to green-light emission (see figure and inside cover).

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页码：878 / +

页数：6

相关论文

共 29 条

[1] Detection of mitochondrial DNA depletion in living human cells using PicoGreen staining [J].

Ashley, N ;

Harris, D ;

Poulton, J .

EXPERIMENTAL CELL RESEARCH, 2005, 303 (02) :432-446

[2] Interchain vs. intrachain energy transfer in acceptor-capped conjugated polymers [J].

Beljonne, D ;

Pourtois, G ;

Silva, C ;

Hennebicq, E ;

Herz, LM ;

Friend, RH ;

Scholes, GD ;

Setayesh, S ;

Müllen, K ;

Brédas, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :10982-10987

[3] A DNA diagnostic biosensor: development, characterisation and performance [J].

Berney, H ;

West, J ;

Haefele, E ;

Alderman, J ;

Lane, W ;

Collins, JK .

SENSORS AND ACTUATORS B-CHEMICAL, 2000, 68 (1-3) :100-108

[4] PHYSICOCHEMICAL STUDY OF COMPLEXES OF 33258-HOECHST WITH DNA AND NUCLEOHISTONE [J].

BONTEMPS, J ;

HOUSSIER, C ;

FREDERICQ, E .

NUCLEIC ACIDS RESEARCH, 1975, 2 (06) :971-984

[5] Recognition of DNA topology in reactions between plasmid DNA and cationic copolymers [J].

Bronich, TK ;

Nguyen, HK ;

Eisenberg, A ;

Kabanov, AV .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (35) :8339-8343

[6] DNA ADDUCTS FROM CARCINOGENIC AND NONCARCINOGENIC ENANTIOMERS OF BENZO[A]PYRENE DIHYDRODIOL EPOXIDE [J].

CHENG, SC ;

HILTON, BD ;

ROMAN, JM ;

DIPPLE, A .

CHEMICAL RESEARCH IN TOXICOLOGY, 1989, 2 (05) :334-340

[7]

Cosa G, 2001, PHOTOCHEM PHOTOBIOL, V73, P585, DOI 10.1562/0031-8655(2001)073<0585:PPOFDD>2.0.CO

[8]

2

[9] THE GENETIC TOXICOLOGY OF ACRIDINES [J].

FERGUSON, LR ;

DENNY, WA .

MUTATION RESEARCH, 1991, 258 (02) :123-160

[10] A METHOD FOR MEASURING MICROQUANTITIES OF DNA [J].

GIACHE, V ;

PIRAMI, L ;

BECCIOLINI, A .

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1994, 9 (03) :229-232