Conjugated-polymer/DNA interpolyelectrolyte complexes for accurate DNA concentration determination

被引：85

作者：

Hong, JW

Henme, WL

Keller, GE

Rinke, MT

Bazan, GC ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Dept Mat, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

[3] Univ Munster, Inst Phys Chem, D-48149 Munster, Germany

来源：

ADVANCED MATERIALS | 2006年 / 18卷 / 07期

关键词：

D O I：

10.1002/adma.200501605

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Precise determination of DNA concentration without the health risks of intercalating dyes is possible via the optical changes that arise from DNA-induced aggregation of cationic conjugated polyelectrolytes. Addition of DNA to a conjugated electrolyte containing two fluorophores results in aggregation and fluorescence quenching, inducing a measurable and quantifiable shift from blue- to green-light emission (see figure and inside cover).

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页码：878 / +

页数：6

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