Identification and properties of microsatellite markers in tsetse flies Glossina morsitans sensu lato (Diptera: Glossinidae)

被引：38

作者：

Baker, MD ^{[1
]}

Krafsur, ES ^{[1
]}

机构：

[1] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA

来源：

MOLECULAR ECOLOGY NOTES | 2001年 / 1卷 / 04期

关键词：

Glossina morsitans complex; microsatellites; simple sequence repeats;

D O I：

10.1046/j.1471-8278.2001.00087.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic libraries enriched for simple sequence repeats were constructed for Glossina morsitans morsitans, G. in. submorsitans, and G. in. centralis. Sixteen microsatellite markers were isolated from the libraries and evaluated on flies from natural G. m. morsitans populations and other Glossina species in the morsitans and palpalis species groups. The primers amplified appropriate sized DNA fragments in the morsitans and palpalis groups. In G. morsitans s.l., eight of 12 dinucleotide repeats and four of 12 trinucleotide repeats were polymorphic. The polymorphic loci showed a mean 7.5 +/- 4.8 alleles per locus and their mean heterozygosity was 55.8 +/- 7.7%.

引用

页码：234 / 236

页数：3

共 9 条

[1]

Edwards KJ, 1996, BIOTECHNIQUES, V20, P758

[2] MICROSATELLITE VARIATION IN NORTH-AMERICAN POPULATIONS OF DROSOPHILA-MELANOGASTER [J].