Use of signature-tagged mutagenesis in pathogenesis studies

被引：59

作者：

Mecsas, J ^{[1
]}

机构：

[1] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA

来源：

CURRENT OPINION IN MICROBIOLOGY | 2002年 / 5卷 / 01期

关键词：

D O I：

10.1016/S1369-5274(02)00282-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

From 2000-2001, over 10 studies have been completed, using signature-tagged mutagenesis, on a variety of bacterial pathogens. Investigators are examining the attenuated strains in additional in vivo, cell culture or in vitro assays to further characterize and catalog the mutants. Notable advances included screening of the attenuated mutants en masse in other assays, verification that mutant strains are defective for growth in specific host tissues, and identification of potential live-attenuated vaccine candidates. In addition, researchers are using signature-tagged strains to characterize the infection process, which is providing snapshots of bottlenecks and quantitative measurements of bacterial spread from tissue to tissue. This review focuses on themes emerging from signature-tagged mutagenesis studies completed on bacterial pathogens since mid-2000.

引用

页码：33 / 37

页数：5

共 37 条

[11] Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis [J].

Foulongne, V ;

Bourg, G ;

Cazevieille, C ;

Michaux-Charachon, S ;

O'Callaghan, D .

INFECTION AND IMMUNITY, 2000, 68 (03) :1297-1303

[12] Identification of Pasteurella multocida virulence genes in a septicemic mouse model using signature-tagged mutagenesis [J].

Fuller, TE ;

Kennedy, MJ ;

Lowery, DE .

MICROBIAL PATHOGENESIS, 2000, 29 (01) :25-38

[13] Identification of Actinobacillus pleuropneumoniae virulence genes using signature-tagged mutagenesis in a swine infection model [J].

Fuller, TE ;

Martin, S ;

Teel, JF ;

Alaniz, GR ;

Kennedy, MJ ;

Lowery, DE .

MICROBIAL PATHOGENESIS, 2000, 29 (01) :39-51

[14] Adaptation of signature-tagged mutagenesis to Escherichia coli K1 and the infant-rat model of invasive disease [J].

Gonzalez, MD ;

Lichtensteiger, CA ;

Vimr, ER .

FEMS MICROBIOLOGY LETTERS, 2001, 198 (02) :125-128

[15] DELETIONS OF CHROMOSOMAL REGIONS CODING FOR FIMBRIAE AND HEMOLYSINS OCCUR INVITRO AND INVIVO IN VARIOUS EXTRAINTESTINAL ESCHERICHIA-COLI ISOLATES [J].

HACKER, J ;

BENDER, L ;

OTT, M ;

WINGENDER, J ;

LUND, B ;

MARRE, R ;

GOEBEL, W .

MICROBIAL PATHOGENESIS, 1990, 8 (03) :213-225

[16] SIMULTANEOUS IDENTIFICATION OF BACTERIAL VIRULENCE GENES BY NEGATIVE SELECTION [J].

HENSEL, M ;

SHEA, JE ;

GLEESON, C ;

JONES, MD ;

DALTON, E ;

HOLDEN, DW .

SCIENCE, 1995, 269 (5222) :400-403

[17] Identification of genes required for chronic persistence of Brucella abortus in mice [J].

Hong, PC ;

Tsolis, RM ;

Ficht, TA .

INFECTION AND IMMUNITY, 2000, 68 (07) :4102-4107

[18] Identification of Streptococcus agalactiae virulence genes in the neonatal rat sepsis model using signature-tagged mutagenesis [J].

Jones, AL ;

Knoll, KM ;

Rubens, CE .

MOLECULAR MICROBIOLOGY, 2000, 37 (06) :1444-1455

[19] Application of high-density array-based signature-tagged mutagenesis to discover novel Yersinia virulence-associated genes [J].

Karlyshev, AV ;

Oyston, PCF ;

Williams, K ;

Clark, GC ;

Titball, RW ;

Winzeler, EA ;

Wren, BW .

INFECTION AND IMMUNITY, 2001, 69 (12) :7810-7819

[20] LARGE, UNSTABLE INSERTS IN THE CHROMOSOME AFFECT VIRULENCE PROPERTIES OF UROPATHOGENIC ESCHERICHIA-COLI O6 STRAIN 536 [J].

KNAPP, S ;

HACKER, J ;

JARCHAU, T ;

GOEBEL, W .

JOURNAL OF BACTERIOLOGY, 1986, 168 (01) :22-30

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