Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents

被引：94

作者：

Gouda, MD ^{[1
]}

Kumar, MA ^{[1
]}

Thakur, MS ^{[1
]}

Karanth, NG ^{[1
]}

机构：

[1] Cent Food Technol Res Inst, Cent Instrument Facil & Serv, Mysore 570013, Karnataka, India

来源：

BIOSENSORS & BIOELECTRONICS | 2002年 / 17卷 / 6-7期

关键词：

biosensor; immobilized enzymes; operational stability; protein based stabilizing agents; detachable membrane unit;

D O I：

10.1016/S0956-5663(02)00021-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

With the incorporation of lysozyme during the immobilization step, considerable enhancement of the operational stability of a biosensor has been demonstrated in the case of an immobilized single enzyme (glucose oxidase) system for glucose and multienzyme (invertase, mutarotase and glucose oxidase) system for sucrose. Thus an increased number of repeated analyses of 750 samples during 230 days for glucose and 400 samples during 40 days of operation for sucrose have been achieved. The increased operational stability of immobilized single and multienzyme system, will improve the operating cost effectiveness of the biosensor. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：503 / 507

页数：5

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