Comparative analysis on microbial and rat metabolism of ginsenoside Rb1 by high-performance liquid chromatography coupled with tandem mass spectrometry

被引：52

作者：

Chen, Guangtong ^{[1
,2
]}

Yang, Min ^{[1
]}

Song, Yan ^{[2
]}

Lu, Zhiqiang ^{[1
]}

Zhang, Jinqiang ^{[1
]}

Huang, Huilian ^{[1
]}

Guan, Shuhong ^{[1
]}

Wu, Lijun ^{[2
]}

Guo, De-an ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China

[2] Shenyang Pharmaceut Univ, Coll Tradit Chinese Mat Med, Shenyang 110016, Liaoning, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2008年 / 22卷 / 07期

关键词：

ginsenoside Rb-1; biotransformation; metabolism; HPLC-MS/MS;

D O I：

10.1002/bmc.1001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ginsenoside Rb-1 is an active protopanaxadiol saponin from Partax species. In order to compare the similarities and differences of microbial and mammalian metabolisms of ginsenoside Rb-1 the microbial transformation by Acremonium strictum and metabolism in rats were comparatively studied. Microbial transformation of ginsenoside Rb-1 by Acremonium strictum AS 3.2058 resulted in the formation of eight metabolites. Ten metabolites (M1-M10) were detected from the in vivo study in rats and eight of them were identified as the same compounds as those obtained from microbial metabolism by liquid chromatography-tandem mass spectrometry analysis and comparison with reference standards obtained from microbial metabolism. Their structures were identified as ginsenoside Rd, gypenoside XVII, 20(S)-ginsenoside Rg(3), 20(R)-ginsenoside Rg(3), ginsenoside F-2, compound K, 12 beta-hydroxydammar-3-one-20(S)-O-beta-D-glucopyranoside, and 25-hydroxyl-(E)-20(22)-ene-ginsenoside Rg(3), respectively. The structures of the additional two metabolites were tentatively characterized as 20(22),24-diene-ginsenoside Rg(3), and 25-hydroxyginsenoside Rd by HPLC-MS/MS analysis. M7-M10 are the first four reported metabolites in vivo. The time course of rat metabolism of ginsenoside Rb-1 was also investigated. Copyright (c) 2008 John Wiley & Sons, Ltd.

引用

页码：779 / 785

页数：7

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