A generic approach to monofunctionalized protein-like gold nanoparticles based on immobilized metal ion affinity chromatography

被引：59

作者：

Lévy, R

Wang, ZX

Duchesne, L

Doty, RC

Cooper, AI

Brust, M

Fernig, DG

机构：

[1] Univ Liverpool, Dept Chem, Sch Biol Sci, Ctr Nanoscale Sci, Liverpool L69 7ZB, Merseyside, England

[2] Changchun Inst Appl Chem, State Key Lab Electroanal, Changchun 130022, Peoples R China

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

gold; IMAC; monofunctionalization; nanoparticles; peptides;

D O I：

10.1002/cbic.200500457

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Control of a peptide-capped gold-nanoparticle (NP) surface with single-molecule accuracy is demonstrated. Immobilized metal ion affinity chromatography (IMAC) has been used to separate peptide-capped NPs as a function of the number of molecular labels (see scheme). The method described in this paper is simple, quantitative and directly applicable to the preparation of monofunctionalized nanoparticles with any water-soluble chemical moieties. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：592 / 594

页数：3

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