Phospholipid metabolism and resistance to glucocorticoid-induced apoptosis in a human leukemic cell line:: a 31P-NMR study using a phosphonium analog of choline

被引：5

作者：

Adebodun, F ^{[1
]}

机构：

[1] N Carolina Agr & Tech State Univ, Dept Chem, Greensboro, NC 27411 USA

来源：

CANCER LETTERS | 1999年 / 140卷 / 1-2期

关键词：

glucocorticoid resistance; apoptosis; phospholipid metabolism; phosphonium analog of choline; leukemic cell lines; nuclear magnetic resonance;

D O I：

10.1016/S0304-3835(99)00085-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In order to evaluate any connection between phospholipid metabolism and the dexamethasone resistance in CEM-C1-15 leukemic cells, a phosphonium analog of choline was used to study the phospholipid metabolism in dexamethasone-sensitive and dexamethasone-resistant leukemic cells using P-31-NMR spectroscopy. Measurements were done on the two cell lines in the presence and absence of dexamethasone, a synthetic glucocorticoid steroid. Dexamethasone was found to cause a significant reduction in the phospholipid metabolism of the dexamethasone-sensitive CEM-C7-14 cells. In contrast, dexamethasone caused a significant enhancement of the phospholipid metabolism in the dexamethasone-resistant CEM-C1-15 cells, indicating a significant difference between the two cell lines in phospholipid metabolism during apoptosis. The results of this study suggest the involvement of phospholipid metabolism in the mechanism of dexamethasone resistance in the CEM-C1-15 cells. (C) 1999 Published by Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：189 / 194

页数：6

共 36 条

[21] CHANGES IN PHOSPHATIDYLETHANOLAMINE METABOLISM IN REGENERATING RAT-LIVER AS MEASURED BY P-31-NMR [J].

MURPHY, EJ ;

BRINDLE, KM ;

RORISON, CJ ;

DIXON, RM ;

RAJAGOPALAN, B ;

RADDA, GK .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1135 (01) :27-34

[22] GENERAL FEATURES OF STEROID RESISTANCE IN LYMPHOID-CELL LINES [J].

PFAHL, M ;

KELLEHER, RJ ;

BOURGEOIS, S .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1978, 10 (02) :193-207

[23] P-31 NUCLEAR-MAGNETIC-RESONANCE STUDIES OF GROWTH-INHIBITION AND DEXAMETHASONE RESISTANCE IN HUMAN LEUKEMIC-CELLS [J].

POST, JFM ;

BAUM, E .

CANCER LETTERS, 1990, 51 (02) :157-162

[24] C-13 NMR-STUDIES OF GLUCOSE-METABOLISM IN HUMAN LEUKEMIC CEM-C7 CELL AND CEM-C1 CELL [J].

POST, JFM ;

BAUM, E ;

EZELL, EL .

MAGNETIC RESONANCE IN MEDICINE, 1992, 23 (02) :356-366

[25] NMR-STUDIES OF PHOSPHOLIPID-METABOLISM AND CELL-PROLIFERATION [J].

RADDA, GK ;

DIXON, RM ;

WOOD, CA .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1991, 19 (04) :995-996

[26] SOCIAL CONTROLS ON CELL-SURVIVAL AND CELL-DEATH [J].

RAFF, MC .

NATURE, 1992, 356 (6368) :397-400

[27]

SELLINS KS, 1987, J IMMUNOL, V139, P3199

[28] METABOLISM OF PHOSPHONIUM ANALOGUE OF CHOLINE IN CULTURED-CELLS - USEFUL NUCLEAR-MAGNETIC-RESONANCE PROBE FOR MEMBRANE PHOSPHATIDYLCHOLINE [J].

SIM, E ;

PASTERNAK, CA .

BIOCHEMICAL JOURNAL, 1976, 154 (01) :105-111

[29] Study of the metabolism of choline and phosphatidylcholine in tumors in vivo using phosphonium-choline [J].

Street, JC ;

Szwergold, BS ;

Matei, C ;

Kappler, F ;

Mahmood, U ;

Brown, TR ;

Koutcher, JA .

MAGNETIC RESONANCE IN MEDICINE, 1997, 38 (05) :769-775

[30] NMR-SPECTROSCOPY OF CELLS [J].

SZWERGOLD, BS .

ANNUAL REVIEW OF PHYSIOLOGY, 1992, 54 :775-798

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