3′-Oligonucleotides conjugation via chemoselective oxime bond formation

被引：35

作者：

Forget, D ^{[1
]}

Renaudet, O ^{[1
]}

Boturyn, D ^{[1
]}

Defrancq, E ^{[1
]}

Dumy, P ^{[1
]}

机构：

[1] Univ Grenoble 1, LEDSS, CNRS, UMR 5616, F-38041 Grenoble 9, France

来源：

TETRAHEDRON LETTERS | 2001年 / 42卷 / 52期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1016/S0040-4039(01)02017-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Chemoselective oxime ligation of cyclopeptide, fluorescein and mannose derivatives at the 3'-end of an oligonucleotide was achieved. The conjugation was performed by reacting oxyamine containing reporter groups to an oligonucleotide bearing an aldehyde at the 3'-extremity. The aldehyde was generated by mild periodate oxidation of a 1,2-aminoalcohol which was readily incorporated at the 3'-end by automated DNA synthesis using the corresponding commercially available support. The straightforward chemical access, their stability in biological media as well as their unchanged hybridisation properties emphasise the interest of such 3'-conjugates. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：9171 / 9174

页数：4

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