Determination of 5′-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence of highly conserved sequence motifs

被引：42

作者：

Oleksiewicz, MB ^{[1
]}

Botner, A ^{[1
]}

Nielsen, J ^{[1
]}

Storgaard, T ^{[1
]}

机构：

[1] Minist Food Agr & Fisheries, Danish Vet Inst Virus Res, DK-4771 Kalvehave, Denmark

来源：

ARCHIVES OF VIROLOGY | 1999年 / 144卷 / 05期

关键词：

D O I：

10.1007/s007050050560

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We determined the untranslated 5'-leader sequence for three different isolates of porcine reproductive and respiratory syndrome virus (PRRSV): pathogenic European- and American-types, as well as an American-type vaccine strain. 5'-leader from European- and American-type PRRSV differed in length (220 and 190 nt, respectively), and exhibited only approximately 50% nucleotide homology. Nevertheless, highly conserved areas were identified in the leader of all 3 PRRSV isolates, which constitute candidate motifs for binding of protein(s) involved in viral replication. These comparative data provide a priori knowledge for mutational identification of virulence determinants in the 5' nontranslated part of the PRRSV genome.

引用

页码：981 / 987

页数：7

共 23 条

[1] AN IN VITRO SYSTEM FOR THE LEADER-PRIMED TRANSCRIPTION OF CORONAVIRUS MRNAS [J].

BAKER, SC ;

LAI, MMC .

EMBO JOURNAL, 1990, 9 (12) :4173-4179

[2] CHARACTERIZATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME (SIRS) VIRUS (ISOLATE ATCC VR-2332) [J].

BENFIELD, DA ;

NELSON, E ;

COLLINS, JE ;

HARRIS, L ;

GOYAL, SM ;

ROBISON, D ;

CHRISTIANSON, WT ;

MORRISON, RB ;

GORCYCA, D ;

CHLADEK, D .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (02) :127-133

[3] ISOLATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUS IN A DANISH SWINE HERD AND EXPERIMENTAL-INFECTION OF PREGNANT GILTS WITH THE VIRUS [J].

BOTNER, A ;

NIELSEN, J ;

BILLEHANSEN, V .

VETERINARY MICROBIOLOGY, 1994, 40 (3-4) :351-360

[4] Appearance of acute PRRS-like symptoms in sow herds after vaccination with a modified live PRRS vaccine [J].

Botner, A ;

Strandbygaard, B ;

Sorensen, KJ ;

Have, P ;

Madsen, KG ;

Madsen, ES ;

Alexandersen, S .

VETERINARY RECORD, 1997, 141 (19) :497-499

[5] INCREASED NEUROVIRULENCE ASSOCIATED WITH A SINGLE NUCLEOTIDE CHANGE IN A NONCODING REGION OF THE SABIN TYPE-3 POLIOVACCINE GENOME [J].

EVANS, DMA ;

DUNN, G ;

MINOR, PD ;

SCHILD, GC ;

CANN, AJ ;

STANWAY, G ;

ALMOND, JW ;

CURREY, K ;

MAIZEL, JV .

NATURE, 1985, 314 (6011) :548-550

[6] A 68-nucleotide sequence within the 3′ noncoding region of simian hemorrhagic fever virus negative-strand RNA binds to four MA104 cell proteins [J].

Hwang, YK ;

Brinton, MA .

JOURNAL OF VIROLOGY, 1998, 72 (05) :4341-4351

[7] Transactivation of the human keratin 4 and Epstein-Barr virus ED-L2 promoters by gut-enriched Kruppel-like factor [J].

Jenkins, TD ;

Opitz, OG ;

Okano, J ;

Rustgi, AK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) :10747-10754

[8] ATTENUATION OF VENEZUELAN EQUINE ENCEPHALITIS-VIRUS STRAIN TC-83 IS ENCODED BY THE 5'-NONCODING REGION AND THE E2 ENVELOPE GLYCOPROTEIN [J].

KINNEY, RM ;

CHANG, GJ ;

TSUCHIYA, KR ;

SNEIDER, JM ;

ROEHRIG, JT ;

WOODWARD, TM ;

TRENT, DW .

JOURNAL OF VIROLOGY, 1993, 67 (03) :1269-1277

[9] REQUIREMENT OF THE 5'-END GENOMIC SEQUENCE AS AN UPSTREAM CIS-ACTING ELEMENT FOR CORONAVIRUS SUBGENOMIC MESSENGER-RNA TRANSCRIPTION [J].

LIAO, CL ;

LAI, MMC .

JOURNAL OF VIROLOGY, 1994, 68 (08) :4727-4737

[10] Sequence analysis of porcine reproductive and respiratory syndrome virus of the American type collected from Danish swine herds [J].

Madsen, KG ;

Hansen, CM ;

Madsen, ES ;

Strandbygaard, B ;

Botner, A ;

Sorensen, KJ .

ARCHIVES OF VIROLOGY, 1998, 143 (09) :1683-1700

← 1 2 3 →