Comparison of three GC/MS methodologies for the analysis of fatty acids in Sinorhizobium meliloti:: Development of a micro-scale, one-vial method

被引:38
作者
Basconcillo, Libia Saborido [1 ]
McCarry, Brian E. [1 ]
机构
[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 871卷 / 01期
关键词
fatty acid methyl esters; Sinorhizobium meliloti; cyclopropane fatty acids; 3-hydroxy fatty acids; 3-hydroxybutanoic acid; poly(3-hydroxybutanoate); hydrolysis;
D O I
10.1016/j.jchromb.2008.06.041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Three protocols for fatty acid analysis in Sinorhizobium meliloti were improved by the addition of a number of standards/controls and a silylation step which allowed the determination of recoveries, extents of conversion of lipids to fatty acid methyl esters (FAMEs) and extents of side reactions. Basic hydrolysis followed by acid-catalyzed methylation and transmethylation with sodium methoxide, were the best for the analysis of 3-hydroxy- and cyclopropane fatty acids, respectively. A micro-scale, one-vial method that employed sodium methoxide/methanol was equally efficient and on a 1000-fold smaller scale than standard methods. Because this method avoids aqueous extractions, 3-hydroxybutanoic acid was detected as its trimethylsilyloxy methyl ester along with FAMEs. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:22 / 31
页数:10
相关论文
共 41 条
[1]   Automated GC-MS analysis of raw biological samples - Application to fatty acid profiling of aquatic micro-organisms [J].
Akoto, L ;
Pel, R ;
Irth, H ;
Brinkman, UAT ;
Vreuls, RJJ .
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS, 2005, 73 (01) :69-75
[2]   Analysis of carboxylic acids in biological fluids by capillary electrophoresis [J].
Baena, B ;
Cifuentes, A ;
Barbas, C .
ELECTROPHORESIS, 2005, 26 (13) :2622-2636
[3]   Development of fatty acid analysis by high-performance liquid chromatography, gas chromatography, and related techniques [J].
Brondz, I .
ANALYTICA CHIMICA ACTA, 2002, 465 (1-2) :1-37
[4]   Development in lipid analysis:: Some new extraction techniques and in situ transesterification [J].
Carrapiso, AI ;
García, C .
LIPIDS, 2000, 35 (11) :1167-1177
[5]   Preparation of fatty acid methyl esters for gas-chromatographic analysis of marine lipids: Insight studies [J].
Carvalho, AP ;
Malcata, FX .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (13) :5049-5059
[6]   A Sinorhizobium meliloti minE mutant has an altered morphology and exhibits defects in legume symbiosis [J].
Cheng, Jiujun ;
Sibley, Christopher D. ;
Zaheer, Rahat ;
Finan, Turlough M. .
MICROBIOLOGY-SGM, 2007, 153 :375-387
[7]  
Christie W.W., 2003, LIPID ANAL ISOLATION, P212
[8]  
CHRISTIE WW, PREPARATION ESTER DE
[9]  
CHRISTIE WW, METHYLATION FATTY AC
[10]   Fatty acid content and juice characteristics in black currant (Ribes nigrum L.) genotypes [J].
del Castillo, MLR ;
Dobson, G ;
Brennan, R ;
Gordon, S .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (04) :948-952