Polarization-based oxygen sensor

被引：23

作者：

Gryczynski, I

Gryczynski, Z

Rao, G

Lakowicz, JR

机构：

[1] Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Ctr Fluorescence Spect, Baltimore, MD 21201 USA

[2] Univ Maryland, Maryland Biotechnol Inst, Ctr Med Biotechnol, Baltimore, MD 21201 USA

[3] Univ Maryland, Maryland Biotechnol Inst, Dept Chem & Biochem Engn, Baltimore, MD 21201 USA

来源：

ANALYST | 1999年 / 124卷 / 07期

关键词：

D O I：

10.1039/a900364i

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A new approach to oxygen sensing based on the luminescence polarization observed from a novel type of sensor is described. The oxygen sensor consists of an oxygen-sensitive silicone film containing tris(4,7-diphenyl-1,10-phenanthroline) chloride [Ru(dpp)(3)Cl-2]and an oxygen-insensitive film of Styryl 7 in poly(vinyl alcohol). Polarizers are used to select orthogonally polarized emission components from Ru(dpp)(3)Cl-2 and Styryl 7. The polarization of the combined emission was found to be highly sensitive to the partial pressure of oxygen. This method of polarization sensing is generic and can be used with any fluorophore which displays an analyte-dependent change in intensity.

引用

页码：1041 / 1044

页数：4

共 28 条

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[2]

[Anonymous], 1991, FIBER OPTIC CHEM SEN

[3]

DETERMINATION OF OXYGEN CONCENTRATIONS BY LUMINESCENCE QUENCHING OF A POLYMER-IMMOBILIZED TRANSITION-METAL COMPLEX [J].