Use of N-[5-(5,7-dimethyl boron dipyrromethene difluoride-sphingomyelin to study membrane traffic along the endocytic pathway

被引：15

作者：

Pagano, RE ^{[1
]}

Watanabe, R ^{[1
]}

Wheatley, C ^{[1
]}

Chen, CS ^{[1
]}

机构：

[1] Mayo Clin & Mayo Fdn, Dept Biochem & Mol Biol, Thorac Dis Res Unit, Rochester, MN 55905 USA

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1999年 / 102卷 / 1-2期

关键词：

fluorescence microscopy; fluorescent lipids; BODIPY (TM); membrane dynamics; endosomes;

D O I：

10.1016/S0009-3084(99)00075-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used N-[5-(5,7-dimethyl boron dipyrromethene difluoride)-1-pentanoyl]-D-erythro-sphingosylphosphorylcholine (C-5-DMB-SM or 'BODIPY(TM)-SM'), a fluorescent analog of sphingomyelin, to study lipid transport along the endocytic pathway of human skin fibroblasts. The unique spectral properties of the BODIPY(TM) fluorophore allow the investigator to distinguish various populations of labeled endosomes and lysosomes within the living cell by fluorescence microscopy, and in conjunction with quantitative fluorescence microscopy, to estimate the concentration of these lipids in different intracellular compartments. This methodology is also applicable for visualizing the accumulation of lipids in the endosomes and lysosomes of storage disease fibroblasts. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：55 / 63

页数：9

共 33 条

[1] Regulated endocytosis of G-protein-coupled receptors by a biochemically and functionally distinct subpopulation of clathrin-coated pits [J].

Cao, TT ;

Mays, RW ;

von Zastrow, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (38) :24592-24602

[2] PARALLAX METHOD FOR DIRECT MEASUREMENT OF MEMBRANE PENETRATION DEPTH UTILIZING FLUORESCENCE QUENCHING BY SPIN-LABELED PHOSPHOLIPIDS [J].

CHATTOPADHYAY, A ;

LONDON, E .

BIOCHEMISTRY, 1987, 26 (01) :39-45

[3] Changes in the spectral properties of a plasma membrane lipid analog during the first seconds of endocytosis in living cells [J].

Chen, CS ;

Martin, OC ;

Pagano, RE .

BIOPHYSICAL JOURNAL, 1997, 72 (01) :37-50

[4] TRAFFICKING OF GLYCOSPHINGOLIPIDS IN EUKARYOTIC CELLS - SORTING AND RECYCLING OF LIPIDS [J].

HOEKSTRA, D ;

KOK, JW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1113 (3-4) :277-294

[5] FLUORESCENT MEMBRANE PROBES INCORPORATING DIPYRROMETHENEBORON DIFLUORIDE FLUOROPHORES [J].

JOHNSON, ID ;

KANG, HC ;

HAUGLAND, RP .

ANALYTICAL BIOCHEMISTRY, 1991, 198 (02) :228-237

[6] Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching [J].

Kaiser, RD ;

London, E .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1375 (1-2) :13-22

[7]

Kang H.C., 1989, PROC SPIE, V1063, P68

[8] FLUORESCENCE AND ABSORPTION SPECTROSCOPIC PROPERTIES OF DIPYRROMETHENEBORON DIFLUORIDE (BODIPY) DERIVATIVES IN LIQUIDS, LIPID-MEMBRANES, AND PROTEINS [J].

KAROLIN, J ;

JOHANSSON, LBA ;

STRANDBERG, L ;

NY, T .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (17) :7801-7806

[9] SALVAGE OF GLUCOSYLCERAMIDE BY RECYCLING AFTER INTERNALIZATION ALONG THE PATHWAY OF RECEPTOR-MEDIATED ENDOCYTOSIS [J].

KOK, JW ;

ESKELINEN, S ;

HOEKSTRA, K ;

HOEKSTRA, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (24) :9896-9900

[10] SORTING OF SPHINGOLIPIDS IN THE ENDOCYTIC PATHWAY OF HT29 CELLS [J].

KOK, JW ;

BABIA, T ;

HOEKSTRA, D .

JOURNAL OF CELL BIOLOGY, 1991, 114 (02) :231-239

← 1 2 3 4 →