Use of N-[5-(5,7-dimethyl boron dipyrromethene difluoride-sphingomyelin to study membrane traffic along the endocytic pathway

被引：15

作者：

Pagano, RE ^{[1
]}

Watanabe, R ^{[1
]}

Wheatley, C ^{[1
]}

Chen, CS ^{[1
]}

机构：

[1] Mayo Clin & Mayo Fdn, Dept Biochem & Mol Biol, Thorac Dis Res Unit, Rochester, MN 55905 USA

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1999年 / 102卷 / 1-2期

关键词：

fluorescence microscopy; fluorescent lipids; BODIPY (TM); membrane dynamics; endosomes;

D O I：

10.1016/S0009-3084(99)00075-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used N-[5-(5,7-dimethyl boron dipyrromethene difluoride)-1-pentanoyl]-D-erythro-sphingosylphosphorylcholine (C-5-DMB-SM or 'BODIPY(TM)-SM'), a fluorescent analog of sphingomyelin, to study lipid transport along the endocytic pathway of human skin fibroblasts. The unique spectral properties of the BODIPY(TM) fluorophore allow the investigator to distinguish various populations of labeled endosomes and lysosomes within the living cell by fluorescence microscopy, and in conjunction with quantitative fluorescence microscopy, to estimate the concentration of these lipids in different intracellular compartments. This methodology is also applicable for visualizing the accumulation of lipids in the endosomes and lysosomes of storage disease fibroblasts. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：55 / 63

页数：9