Dorsoventral patterning in xenopus: Inhibition of ventral signals by direct binding of Chordin to BMP-4

被引：909

作者：

Piccolo, S

Sasai, Y

Lu, B

DeRobertis, EM

机构：

[1] Department of Biological Sciences, Kyoto Univ. Faculty of Medicine

[2] Howard Hughes Medical Institute, Department of Biological Chemistry, University of California, Los Angeles

来源：

CELL | 1996年 / 86卷 / 04期

关键词：

D O I：

10.1016/S0092-8674(00)80132-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chordin (Chd) is an abundant protein secreted by Spemann organizer tissue during gastrulation. Chd antagonizes signaling by mature bone morphogenetic proteins (BMPs) by blocking binding to their receptors. Recombinant Xenopus Chd binds to BMP-4 with high affinity (K-D 3 x 10(-10) M), binding specifically to BMPs but not to activin or TGF-beta 1. Chd protein is able to dorsalize mesoderm and to neuralize ectoderm in Xenopus gastrula explants at 1 nM. We propose that the noncell-autonomous effects of Spemann's organizer on dorsoventral patterning are executed in part by diffusible signals that directly bind to and neutralize ventral BMPs during gastrulation.

引用

页码：589 / 598

页数：10

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