Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells

被引：83

作者：

Christian, AT ^{[1
]}

Pattee, MS ^{[1
]}

Attix, CM ^{[1
]}

Reed, BE ^{[1
]}

Sorensen, KJ ^{[1
]}

Tucker, JD ^{[1
]}

机构：

[1] Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94551 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 25期

关键词：

D O I：

10.1073/pnas.251383598

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rolling circle amplification has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate that rolling circle amplification in situ can detect gene copy number and single base mutations in fixed cells with efficiencies up to 90%. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.

引用

页码：14238 / 14243

页数：6

共 13 条

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Zhong, XB
Lizardi, PM
Huang, XH
Bray-Ward, PL
Ward, DC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (07) : 3940 - 3945

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