Formation and dissolution of hen egg white lysozyme amyloid fibrils in protic ionic liquids

被引：94

作者：

Byrne, Nolene ^{[1
]}

Angell, C. Austen ^{[1
]}

机构：

[1] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

来源：

CHEMICAL COMMUNICATIONS | 2009年 / 09期

关键词：

PROTEIN; AGGREGATION; DISEASE;

D O I：

10.1039/b817590j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The formation of amyloid fibrils from non-disease-related proteins demonstrates that any protein can adopt this "rogue'' form; we show that it is possible to use protic ionic liquids to fibrilize hen egg white lysozyme, and then subsequently to dissolve the fibrils with up to 72% restoration of enzymatic activity.

引用

页码：1046 / 1048

页数：3

共 31 条

[21] 2 SIMPLE METHODS FOR QUANTIFYING LOW-AFFINITY DYE SUBSTRATE BINDING [J].

KLUNK, WE ;

PETTEGREW, JW ;

ABRAHAM, DJ .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1989, 37 (08) :1293-1297

[22] Enzyme catalysis in ionic liquids [J].

Kragl, U ;

Eckstein, M ;

Kaftzik, N .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (06) :565-571

[23] Ionic liquids as refolding additives:: N′-alkyl and N′-(ω-hydroxyalkyl) N-methylimidazolium chlorides [J].

Lange, C ;

Patil, G ;

Rudolph, R .

PROTEIN SCIENCE, 2005, 14 (10) :2693-2701

[24]

LeVine H, 1999, METHOD ENZYMOL, V309, P274

[25] THIOFLAVINE-T INTERACTION WITH SYNTHETIC ALZHEIMERS-DISEASE BETA-AMYLOID PEPTIDES - DETECTION OF AMYLOID AGGREGATION IN SOLUTION [J].

LEVINE, H .

PROTEIN SCIENCE, 1993, 2 (03) :404-410

[26] Protein crystallization using room temperature ionic liquids [J].

Pusey, Marc L. ;

Paley, Mark Steve ;

Turner, Megan B. ;

Rogers, Robin D. .

CRYSTAL GROWTH & DESIGN, 2007, 7 (04) :787-793

[27] Heat-induced conversion of β2-Microglobulin and hen egg-white lysozyme into amyloid fibrils [J].

Sasahara, Kenji ;

Yagi, Hisashi ;

Naiki, Hironobu ;

Goto, Yuji .

JOURNAL OF MOLECULAR BIOLOGY, 2007, 372 (04) :981-991

[28] THE MEASUREMENT OF LYSOZYME ACTIVITY AND THE ULTRA-VIOLET INACTIVATION OF LYSOZYME [J].

SHUGAR, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1952, 8 (03) :302-309

[29] Protein renaturation by the liquid organic salt ethylammonium nitrate [J].

Summers, CA ;

Flowers, RA .

PROTEIN SCIENCE, 2000, 9 (10) :2001-2008

[30] Dissolution of cellose with ionic liquids [J].

Swatloski, RP ;

Spear, SK ;

Holbrey, JD ;

Rogers, RD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (18) :4974-4975

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