Atomic-force-microscopy imaging and molecular-recognition-force microscopy of recrystallized heterotetramers comprising an S-layer-streptavidin fusion protein

被引：21

作者：

Ebner, A

Kienberger, F

Huber, C

Kamruzzahan, ASM

Pastushenko, VP

Tang, JL

Kada, G

Gruber, HJ

Sleytr, UB

Sára, M

Hinterdorfer, P ^{[1
]}

机构：

[1] Univ Linz, Inst Biophys, A-4040 Linz, Austria

[2] Univ Nat Resources & Appl Life Sci, Ctr NanoBiotechnol, A-1180 Vienna, Austria

[3] Mol Imaging Corp, Tempe, AZ 85282 USA

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 04期

关键词：

AFM; imaging; nanoarrays; recognition; S-layers;

D O I：

10.1002/cbic.200500445

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

S-layer proteins reassemble on surfaces to form 2D crystals with lattice constants in the nanometer range. By modifying these proteins with capture molecules through genetic engineering, specific binding sites, such as streptavidin tags, can be introduced. The activity of these sites is proven by single-molecule recognition-force spectroscopy. In addition, structural details of the morphological units were resolved by using dynamic force microscopy imaging. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：588 / 591

页数：4

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