Polymorphism of flagellin A gene in Helicobacter pylori

AIM: To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori). METHODS: As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program. RESULTS: Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and! library sequences derived from different H. pylori, strains: were higher than 90%. CONCLUSION: The nucleotide sequence of flagellin A in H., pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.