miR-1289 and "Zipcode"-like Sequence Enrich mRNAs in Microvesicles

被引：225

作者：

Bolukbasi, Mehmet Fatih ^{[1
]}

Mizrak, Arda ^{[1
]}

Ozdener, Gokhan Baris ^{[1
]}

Madlener, Sibylle ^{[2
]}

Stroebel, Thomas ^{[3
]}

Erkan, Erdogan Pekcan ^{[2
]}

Fan, Jian-Bing ^{[4
]}

Breakefield, Xandra O. ^{[1
]}

Saydam, Okay ^{[2
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Neurol & Radiol, Boston, MA USA

[2] Med Univ Vienna, Dept Pediat, Div Oncol, Mol Neurooncol Res Unit, A-1090 Vienna, Austria

[3] Med Univ Vienna, Inst Neurol, A-1090 Vienna, Austria

[4] Illumina Inc, San Diego, CA USA

来源：

MOLECULAR THERAPY-NUCLEIC ACIDS | 2012年 / 1卷

关键词：

D O I：

10.1038/mtna.2011.2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Despite intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles (MVs) are still unknown. This is the first study describing a zipcode-like 25 nucleotide (nt) sequence in the 3'-untranslated region (3'UTR) of mRNAs, with variants of this sequence present in many mRNAs enriched in MVs, as compared to their glioblastoma cells of origin. When this sequence was incorporated into the 3' UTR of a reporter message and expressed in a different cell type, it led to enrichment of the reporter mRNA in MVs. Critical features of this sequence are both a CUGCC core presented on a stemloop structure and a miRNA-binding site, with increased levels of the corresponding miRNA in cells further increasing levels of mRNAs in MVs.

引用

页数：10

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