The Ba813 chromosomal DNA sequence effectively traces the whole Bacillus anthracis community

被引：64

作者：

Ramisse, V

Patra, G

Vaissaire, J

Mock, M

机构：

[1] Ctr Etud Bouchet, Appl Microbiol Lab, F-91710 Vert Le Petit, France

[2] Univ Scranton, Inst Mol Biol & Med, Scranton, PA 18510 USA

[3] Inst Pasteur, Unite Toxines & Pathogenies Bacteriennes, Paris, France

[4] CNEVA Alfort, Lab Cent Rech Vet, Unite Zoonoses Bacteriennes, Maisons Alfort, France

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 1999年 / 87卷 / 02期

关键词：

D O I：

10.1046/j.1365-2672.1999.00874.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plasmid genes that are responsible for virulence of Bacillus anthparcis are important targets for the DNA-based detection of anthrax. We evaluated the distribution of the Ba813 chromosomal DNA sequence (Ba813) within closely related Bacillus species. Ba813 was systematically identified from 47 strains or isolates of B, anthracis tested, thus indicating its reliability: as a tracer for that species. From the 60 strains of closely related Bacillus spp, examined, three bona fide B. cer-eus and one bona fide B. thuringiensis were found to harbour Ba813. This marker was also detected in Bacillus sp. isolates that were present at high levels in soil samples collected in a place where an anthrax outbreak had occurred. The significance and the possible function of the Ba813 locus is discussed.

引用

页码：224 / 228

页数：5

共 30 条

[1] Identification of a region of genetic variability among Bacillus anthracis strains and related species [J].