Biosynthesis of 2′-O-methylmyxalamide D in the myxobacterium Cystobacter fuscus:: a polyketide synthase-nonribosomal peptide synthetase system for the myxalamide D skeleton and a methyltransferase for the final O-methylation

被引：2

作者：

Feng, ZY ^{[1
]}

Qi, JH ^{[1
]}

Tsuge, T ^{[1
]}

Oba, Y ^{[1
]}

Sakagami, Y ^{[1
]}

Ojika, M ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 03期

基金：

日本学术振兴会;

关键词：

biosynthetic gene cluster; myxobacterium; antifungal agent; polyketide synthase; methyltransferase;

D O I：

10.1271/bbb.70.699

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biosynthetic gene cluster for the polyene antifungal antibiotic, 2'-O-methylmyxalamide D, was cloned from myxobacterium Cystobacter fuscus AJ-13278. A sequence analysis of the 12.8-kb region in the gene cluster revealed the presence of two type I polyketide synthase genes, mmxB and mmxC. The involvement of these two genes in the biosynthesis of 2'-O-methylmyxalamide D was confirmed by a gene disruption experiments. In addition, an S-adenosylmethionine-dependent methyltransferase gene (mmxM) was found downstream of the gene cluster and demonstrated, by a gene disruption analysis, to be responsible for converting the known unmethylated precursor, myxalamide D, into 2'-O-methylmyxalamide D.

引用

页码：699 / 705

页数：7

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