Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase

被引：660

作者：

Kamura, T

Koepp, DM

Conrad, MN

Skowyra, D

Moreland, RJ

Iliopoulos, O

Lane, WS

Kaelin, WG

Elledge, SJ

Conaway, RC

Harper, JW

Conway, JW

机构：

[1] Oklahoma Med Res Fdn, Howard Hughes Med Inst, Oklahoma City, OK 73104 USA

[2] Oklahoma Med Res Fdn, Howard Hughes Med Inst, Oklahoma City, OK 73104 USA

[3] Oklahoma Med Res Fdn, Program Mol & Cell Biol, Oklahoma City, OK 73104 USA

[4] Baylor Coll Med, Verna & Marrs McLean Dept Biochem, Houston, TX 77030 USA

[5] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[6] Harvard Univ, Brigham & Womens Hosp, Sch Med, Boston, MA 02115 USA

[7] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02115 USA

[8] Harvard Univ, Sch Med, Dana Farber Canc Inst, Boston, MA 02115 USA

[9] Harvard Univ, Harvard Microchem Facil, Cambridge, MA 02138 USA

[10] Univ Oklahoma, Hlth Sci Ctr, Dept Biochem & Mol Biol, Oklahoma City, OK 73190 USA

来源：

SCIENCE | 1999年 / 284卷 / 5414期

关键词：

D O I：

10.1126/science.284.5414.657

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also includes Rbx1, an evolutionarily conserved protein that contains a RING-H2 fingerlike motif and that interacts with Cullins. The yeast homolog of Rbx1 is a subunit and potent activator of the Cdc53-containing SCFCdc4 ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor Sic1 and for the G(1) to S cell cycle transition. These findings provide a further Link between VHL and the cellular ubiquitination machinery.

引用

页码：657 / 661

页数：5