Determination of L-ascorbic acid in wines by direct injection liquid chromatography using a polymeric column

被引：29

作者：

Lopes, P ^{[1
]}

Drinkine, J ^{[1
]}

Saucier, C ^{[1
]}

Glories, Y ^{[1
]}

机构：

[1] Univ Victor Segalen Bordeaux 2, Fac Enol Bordeaux, UMR 1219, INRA, F-33405 Talence, France

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 555卷 / 02期

关键词：

liquid chromatography; L-ascorbic acid; wine;

D O I：

10.1016/j.aca.2005.09.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid method for the identification and quantification of L-ascorbic acid in wines by direct injection liquid chromatography equipped with a UV detection was developed. The levels of ascorbic acid were determined using a polymeric PLRP-S 100 A (5 mu m) column (150 mm x 4.6 mm) with a mobile water/trifluoroacetic acid (99/1, v/v) phase. The method is rapid (less than 5 min) and sensitive (LOQ of 5 mg L(-1)). The calibration curve of ascorbic acid was linear (r = 0.999) over a concentration range between 1 and 200 mg L(-1). Repeatability was less than 2.5% and the recovery over 95%. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：242 / 245

页数：4

共 29 条

[1] Food analyses:: a new calorimetric method for ascorbic acid (vitamin C) determination
Antonelli, ML
D'Ascenzo, G
Laganà, A
Pusceddu, P
[J]. TALANTA, 2002, 58 (05) : 961 - 967
[2] ARAKAWA N, 1981, J NUTR SCI VITAMINOL, V27, P1, DOI 10.3177/jnsv.27.1
[3] Spectrophotometric determination of vitamin C with iron(II)-4-(2-pyridylazo)resorcinol complex
Arya, SP
Mahajan, M
Jain, P
[J]. ANALYTICA CHIMICA ACTA, 2001, 427 (02) : 245 - 251
[4] BEUTLER HO, 1984, METHOD ENZYMAT AN, V6, P376
[5] Defining the ascorbic acid crossover from anti-oxidant to pro-oxidant in a model wine matrix containing (+)-catechin
Bradshaw, MP
Cheynier, V
Scollary, GR
Prenzler, PD
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (14) : 4126 - 4132
[6] Ascorbic acid-induced browning of (+)-catechin in a model wine system
Bradshaw, MP
Prenzler, PD
Scollary, GR
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (02) : 934 - 939
[7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ASCORBIC-ACID IN FRUITS, VEGETABLES AND JUICES
BUSHWAY, RJ
KING, JM
PERKINS, B
KRISHNAN, M
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (16): : 3415 - 3423
[8] Determination of sulfur dioxide and ascorbic acid in beverages using a dual channel flow injection electrochemical detection system
Cardwell, TJ
Christophersen, MJ
[J]. ANALYTICA CHIMICA ACTA, 2000, 416 (01) : 105 - 110
[9] Sensitive spectrophotometric determination of ascorbic acid in fruit juices and pharmaceutical formulations using 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP)
Ferreira, SLC
Bandeira, MLSF
Lemos, VA
dosSantos, HC
Costa, ACS
deJesus, DS
[J]. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1997, 357 (08): : 1174 - 1178
[10] Fabrication and voltammetric study of lanthanum 2,6-dichlorophenolindophenol chemically modified screen printed electrodes. Application for the determination of ascorbic acid
Florou, AB
Prodromidis, MI
Tzouwara-Karayanni, SM
Karayannis, MI
[J]. ANALYTICA CHIMICA ACTA, 2000, 423 (01) : 107 - 114

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