Unstable Polymerase-Nucleoprotein Interaction Is Not Responsible for Avian Influenza Virus Polymerase Restriction in Human Cells

被引：34

作者：

Cauldwell, Anna V. ^{[1
]}

Moncorge, Olivier ^{[1
]}

Barclay, Wendy S. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Infect Dis, London, England

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 02期

基金：

英国惠康基金;

关键词：

HOST-RANGE DETERMINANTS; A VIRUSES; MUTATIONAL ANALYSIS; VIRAL POLYMERASE; MAMMALIAN-CELLS; PANDEMIC H1N1; AMINO-ACID; PB2; H5N1; REPLICATION;

D O I：

10.1128/JVI.02597-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Avian-origin influenza virus polymerase activity can be dramatically increased in human cells with the PB2 E627K mutation. Previously, others have proposed that this mutation increases the stability of the viral ribonucleoprotein complex (vRNP) measured by the interaction between PB2 and NP. However, we demonstrate here that a variety of PB2 adaptive mutations, including E627K, do not enhance the stability of the vRNP but rather increase the amount of replicated RNA that results in more PB2-NP coprecipitation.

引用

页码：1278 / 1284

页数：7

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