Thickness measurements on cell monolayers using CR-39 detectors

被引：11

作者：

Dörschel, B ^{[1
]}

Hermsdorf, D

Pieck, S

Starke, S

Thiele, H

机构：

[1] Dresden Univ Technol, Dept Phys, Inst Radiat Protect Phys, D-01062 Dresden, Germany

[2] Dresden Univ Technol, Inst Zool, Dept Biol, D-01062 Dresden, Germany

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 2002年 / 187卷 / 04期

关键词：

cell thickness; solid state nuclear track detector; alpha particles; track diameter; track length; thickness fluctuations;

D O I：

10.1016/S0168-583X(01)01148-X

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Interpretation of radiation effects on living cells requires the determination of the absorbed dose. Dosimetry in cell monolayers irradiated by charged particles needs the knowledge of the mean cell thickness. Thickness measurement was made utilising the energy alteration of alpha particles traversing the cell monolayers. The reduced energy was determined from diameters or lengths of etched tracks produced by the penetrating alpha particles in CR-39. The experiments were performed using 3T3 cells grown directly on the CR-39 surface. The reliability of the method has been studied by means of a detailed error analysis. The total thickness errors are composed of the measurement uncertainty and the fluctuations of the cell thickness within the monolayers. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：525 / 534

页数：10

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