Simultaneous Analysis of Free Phytosterols/Phytostanols and Intact Phytosteryl/Phytostanyl Fatty Acid and Phenolic Acid Esters in Cereals

被引：57

作者：

Esche, Rebecca ^{[1
]}

Barnsteiner, Andreas ^{[1
]}

Scholz, Birgit ^{[1
]}

Engel, Karl-Heinz ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Allgemeine Lebensmitteltechnol, D-85350 Freising Weihenstephan, Germany

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 21期

关键词：

solid-phase extraction; sterols; steryl fatty acid ester; steryl phenolic acid ester; cereals; PLANT STEROLS; LIQUID-CHROMATOGRAPHY; ANTIOXIDANT ACTIVITY; GAS-CHROMATOGRAPHY; STERYL FERULATE; CORN; PHYTOSTEROLS; WHEAT; IDENTIFICATION; OIL;

D O I：

10.1021/jf300878h

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An approach based on solid-phase extraction for the effective separation of free phytosterols/phytostanols and phytosteryl/phytostanyl fatty acid and phenolic acid esters from cereal lipids was developed. The ester conjugates were analyzed in their intact form by means of capillary gas chromatography. Besides free sterols and stanols, up to 33 different fatty acid and phenolic acid esters were identified in four different cereal grains via gas chromatography-mass spectrometry. The majority (52-57%) of the sterols and stanols were present as fatty acid esters. The highest levels of all three sterol and stanol classes based on dry matter of ground kernels were determined in corn, whereas the oil extract of rye was 1.7 and 1.6 times richer in fatty acid esters and free sterols/stanols than the corn oil. The results showed that there are considerable differences in the sterols/stanols and their ester profiles and contents obtained from corn compared to rye, wheat, and spelt. The proposed method is useful for the quantification of a wide range of free phytosterols/phytostanols and intact phytosteryl/phytostanyl esters to characterize different types of grain.

引用

页码：5330 / 5339

页数：10

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