Enzyme-functionalized mesoporous silica for bioanalytical applications

被引：200

作者：

Ispas, Cristina ^{[1
]}

Sokolov, Igor ^{[2
]}

Andreescu, Silvana ^{[1
]}

机构：

[1] Clarkson Univ, Dept Chem & Biomol Sci, Potsdam, NY 13699 USA

[2] Clarkson Univ, Dept Phys, Potsdam, NY 13699 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 393卷 / 02期

基金：

美国国家科学基金会;

关键词：

Mesoporous silica; Enzyme; Immobilization; Biosensors; CATALYTIC-ACTIVITY; MOLECULAR-SIEVES; CYTOCHROME-C; DIRECT ELECTROCHEMISTRY; AMPEROMETRIC BIOSENSOR; MORPHOLOGICAL CONTROL; IMMOBILIZED ENZYMES; CARBON NANOTUBES; PORE-SIZE; ENCAPSULATION;

D O I：

10.1007/s00216-008-2250-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The unique properties of mesoporous silica materials (MPs) have attracted substantial interest for use as enzyme-immobilization matrices. These features include high surface area, chemical, thermal, and mechanical stability, highly uniform pore distribution and tunable pore size, high adsorption capacity, and an ordered porous network for free diffusion of substrates and reaction products. Research demonstrated that enzymes encapsulated or entrapped in MPs retain their biocatalytic activity and are more stable than enzymes in solution. This review discusses recent advances in the study and use of mesoporous silica for enzyme immobilization and application in biosensor technology. Different types of MPs, their morphological and structural characteristics, and strategies used for their functionalization with enzymes are discussed. Finally, prospective and potential benefits of these materials for bioanalytical applications and biosensor technology are also presented.

引用

页码：543 / 554

页数：12

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