Differential expression of wheat transcriptomes in response to varying cadmium concentrations

被引：16

作者：

Cebeci, O. ^{[1
]}

Kokturk, B. ^{[1
]}

Ergen, N. ^{[1
]}

Ozturk, L. ^{[1
]}

Cakmak, I. ^{[1
]}

Budak, H. ^{[1
]}

机构：

[1] Sabanci Univ, Fac Engn & Nat Sci, Biol Sci & Bio Engn Program, TR-34956 Istanbul, Turkey

来源：

BIOLOGIA PLANTARUM | 2008年 / 52卷 / 04期

关键词：

cadmium-responsive genes; functional genomics; mRNA differential display; Triticum durum;

D O I：

10.1007/s10535-008-0135-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This study aims to understand the changes in the transcriptome of durum wheat (Tricitum durum cv. Balcali-85) upon exposure to varying Cd concentrations using mRNA differential display (mRNA DD) technique. Sequence analyses of the two heavily induced genes upon exposure to Cd showed high homology to NADH dehydrogenase subunit 1 (EC907725) and PsaC gene encoding a photosystem 1 (PS 1) 9 kDa subunit protein (EC907731). Additionally, three differentially expressed genes (EC907726, EC907729 and EC907730) were identified. Their sequence analyses revealed no significant homologies to known genes. The expressions of NADH dehydrogenase subunit 1 and PsaC genes were confirmed by Northern blot analysis and quantified by real time PCR. This is the first report for the induction of NADH dehydrogenase subunit 1 gene during Cd stress in wheat.

引用

页码：703 / 708

页数：6

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