Power-Assisted Liposuction Versus Tissue Resection for the Isolation of Adipose Tissue-Derived Mesenchymal Stem Cells: Phenotype, Senescence, and Multipotency at Advanced Passages

被引:16
作者
Barzelay, Aya [1 ,2 ]
Levy, Ran [1 ,2 ]
Kohn, Emmanulle [1 ,2 ]
Sella, Meirav [4 ]
Shani, Nir
Meilik, Benjamin [4 ]
Entin-Meer, Michal [5 ]
Gur, Eyal [4 ]
Loewenstein, Anat [1 ,2 ]
Barak, Adiel [1 ,2 ,3 ]
机构
[1] Tel Aviv Univ, Tel Aviv Sourasky Med Ctr, Ophthalmol, IL-69978 Tel Aviv, Israel
[2] Tel Aviv Univ, Tel Aviv Sourasky Med Ctr, Dept Ophthalmol, IL-69978 Tel Aviv, Israel
[3] Tel Aviv Univ, Tel Aviv Sourasky Med Ctr, Vitreoretinal Surg Unit, Ophthalmol Lab, IL-69978 Tel Aviv, Israel
[4] Tel Aviv Sourasky Med Ctr, Dept Plast & Reconstruct Surg, Tel Aviv, Israel
[5] Tel Aviv Sourasky Med Ctr, Dept Cardiol, Tel Aviv, Israel
关键词
BONE-MARROW; IN-VITRO; LIPOPLASTY; DIFFERENTIATION; PROGENITORS; ASPIRATION; MORBIDITY; MORTALITY; BLOOD; VIVO;
D O I
10.1093/asj/sjv055
中图分类号
R61 [外科手术学];
学科分类号
100210 [外科学];
摘要
Adipose tissue-derived mesenchymal stem cells (ASCs) can be isolated from subcutaneous fat harvested by tissue resection or liposuction. The authors compared ASCs isolated by tissue resection or power-assisted liposuction (PAL) to determine whether either surgical procedure yielded ASCs with improved purity and competence that was preserved for several passages. For this experimental study, ASCs were isolated from fat harvested by tissue resection or PAL from six patients who underwent abdominoplasty. ASCs were counted to determine cell yields, and viabilities were assessed with an amine-reactive dye and by fluorescence-activated cell sorting (FACS). Cell phenotypes were determined by immunostaining and FACS, and doubling times were calculated. Senescence ratios of the cells were detected by gene profiling and by assaying beta-galactosidase activity. Multipotency was evaluated by induced differentiation analyses. No significant differences were observed in cell numbers or viabilities of ASCs isolated following either surgical method of fat harvesting. Both populations of cultured ASCs expressed markers of mesenchymal stem cells and preserved this expression pattern through the third passage. PAL and tissue resection yielded ASCs with similar division rates, similar senescence ratios into the fourth passage, and similar capacities to differentiate into osteocytes or adipocytes. Fat harvested by PAL or tissue resection yielded uniform cultures of ASCs with high division rates, low senescence ratios, and multipotency preserved into passages 3 and 4. Because PAL is less invasive, it may be preferable for the isolation of ASCs.
引用
收藏
页码:NP230 / NP240
页数:11
相关论文
共 31 条
[1]
Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow [J].
Campagnoli, C ;
Roberts, IAG ;
Kumar, S ;
Bennett, PR ;
Bellantuono, I ;
Fisk, NM .
BLOOD, 2001, 98 (08) :2396-2402
[2]
Lipoaspiration and its complications:: A safe operation [J].
Cárdenas-Camarena, L .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2003, 112 (05) :1435-1441
[3]
Comparison of multi-lineage cells from human adipose tissue and bone marrow [J].
De Ugarte, DA ;
Morizono, K ;
Elbarbary, A ;
Alfonso, Z ;
Zuk, PA ;
Zhu, M ;
Dragoo, JL ;
Ashjian, P ;
Thomas, B ;
Benhaim, P ;
Chen, I ;
Fraser, J ;
Hedrick, MH .
CELLS TISSUES ORGANS, 2003, 174 (03) :101-109
[4]
The secreted glycoprotein CREG inhibits cell growth dependent on the mannose-6-phosphate/insulin-like growth factor II receptor [J].
Di Bacco, A ;
Gill, G .
ONCOGENE, 2003, 22 (35) :5436-5445
[5]
A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].
DIMRI, GP ;
LEE, XH ;
BASILE, G ;
ACOSTA, M ;
SCOTT, C ;
ROSKELLEY, C ;
MEDRANO, EE ;
LINSKENS, M ;
RUBELJ, I ;
PEREIRASMITH, O ;
PEACOCKE, M ;
CAMPISI, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367
[6]
Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo [J].
Erickson, GR ;
Gimble, JM ;
Franklin, DM ;
Rice, HE ;
Awad, H ;
Guilak, F .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 290 (02) :763-769
[7]
LIPO-SUCTION SURGERY - A REVIEW [J].
FIELD, LM ;
ASKEN, S ;
CAVER, RG ;
CHRISMAN, BB ;
COLLINS, PS ;
FARBER, GA ;
GLOGAU, RG ;
NARINS, RS ;
PIERCE, H ;
STEGMAN, SJ ;
SUMRALL, AJ ;
TROMOVITCH, TA .
JOURNAL OF DERMATOLOGIC SURGERY AND ONCOLOGY, 1984, 10 (07) :530-538
[8]
Power-assisted lipoplasty (PAL): A clinical pilot study comparing PAL to traditional lipoplasty (TL) [J].
Fodor, PB ;
Vogt, PA .
AESTHETIC PLASTIC SURGERY, 1999, 23 (06) :379-385
[9]
Critical pathways in cellular senescence and immortalization revealed by gene expression profiling [J].
Fridman, A. L. ;
Tainsky, M. A. .
ONCOGENE, 2008, 27 (46) :5975-5987
[10]
Surface protein characterization of human adipose tissue-derived stromal cells [J].
Gronthos, S ;
Franklin, DM ;
Leddy, HA ;
Robey, PG ;
Storms, RW ;
Gimble, JM .
JOURNAL OF CELLULAR PHYSIOLOGY, 2001, 189 (01) :54-63