Identification of an IgE-binding region in soybean acidic glycinin G1

被引：37

作者：

Zeece, MG ^{[1
]}

Beardslee, TA

Markwell, JP

Sarath, G

机构：

[1] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA

[2] Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 1999年 / 11卷 / 01期

关键词：

IgE-binding proteins; food allergy; glycinin; soybean;

D O I：

10.1080/09540109999942

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The acidic polypeptide chain of soybean glycinin G1 was identified as one of the several IgE-binding proteins by immune-blotting with pooled sera from soy-allergic individuals. The region of IgE-binding within glycinin GI was narrowed by in situ digestion with endoproteinase GluC followed by immune-blotting of peptide products. This procedure resulted in a single IgE-binding fragment of approximately 15 kDa corresponding to residues 192 to 306 of the acidic chain of glycinin G1.

引用

页码：83 / 90

页数：8

共 19 条

[1] Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K [J].

Bando, N ;

Tsuji, H ;

Yamanishi, R ;

Nio, N ;

Ogawa, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (02) :347-348

[2]

BURKS AW, 1991, ADV EXP MED BIOL, V289, P295

[3] ALLERGENICITY OF MAJOR COMPONENT PROTEINS OF SOYBEAN DETERMINED BY ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) AND IMMUNOBLOTTING IN CHILDREN WITH ATOPIC-DERMATITIS AND POSITIVE SOY CHALLENGES [J].

BURKS, AW ;

BROOKS, JR ;

SAMPSON, HA .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1988, 81 (06) :1135-1142

[4]

CLEVELAND DW, 1983, METHOD ENZYMOL, V96, P222

[5]

DJURTOFT R, 1991, ADV EXP MED BIOL, V289, P281

[6] IDENTIFICATION OF SOYBEAN ALLERGENS BY IMMUNOBLOTTING WITH SERA FROM SOY-ALLERGIC ADULTS [J].

HERIAN, AM ;

TAYLOR, SL ;

BUSH, RK .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1990, 92 (02) :193-198

[7]

KALINSKI A, 1992, J BIOL CHEM, V267, P12068

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] MOLECULAR-STRUCTURE AND IMMUNOLOGICAL PROPERTIES OF FOOD ALLERGENS [J].

MATSUDA, T ;

NAKAMURA, R .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1993, 4 (09) :289-293

[10] KUNITZ SOYBEAN TRYPSIN-INHIBITOR - A SPECIFIC ALLERGEN IN FOOD ANAPHYLAXIS [J].

MOROZ, LA ;

YANG, WH .

NEW ENGLAND JOURNAL OF MEDICINE, 1980, 302 (20) :1126-1128

← 1 2 →