Individual RNA Base Recognition in Immobilized Oligonucleotides Using a Protein Nanopore

被引：61

作者：

Ayub, Mariam ^{[1
]}

Bayley, Hagan ^{[1
]}

机构：

[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

来源：

NANO LETTERS | 2012年 / 12卷 / 11期

基金：

美国国家卫生研究院;

关键词：

alpha-hemolysin; nanopore; RNA sequencing modified bases; protein engineering; single-nucleotide discrimination; SINGLE-NUCLEOTIDE RESOLUTION; SOLID-STATE NANOPORES; PHI29; DNA-POLYMERASE; ALPHA-HEMOLYSIN; BIOLOGICAL NANOPORE; POLYNUCLEOTIDE MOLECULES; GENE FUSIONS; WEB SERVER; DISCRIMINATION; TRANSLOCATION;

D O I：

10.1021/nl3027873

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein nanopores are under investigation as key components of rapid, low-cost platforms to sequence DNA molecules. Previously, it has been shown that the alpha-hemolysin (alpha HL) nanopore contains three recognition sites, capable of discriminating between individual DNA bases when oligonucleotides are immobilized Within the nanopore. However, the direct sequencing of RNA is also of critical importance. Here, we achieve sharply defined current distributions that enable clear discrimination of the four nucleobases, guanine, cytosine, adenine, and uracil, in RNA. Further, the Modified bases, inosine N-6-methyladenosine and N-5-methylcytosine can be distinguished.

引用

收藏

页码：5637 / 5643

页数：7

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