Recognizing a single base in an individual DNA strand:: A step toward DNA sequencing in nanopores

被引：164

作者：

Ashkenasy, N

Sánchez-Quesada, J

Bayley, H

Ghadiri, MR

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[4] Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 09期

关键词：

DNA; host-guest chemistry; molecular recognition; rotaxanes; single-molecule studies;

D O I：

10.1002/anie.200462114

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL·DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA. © 2005 Wiley-VCH Verlag GmbH & Co. KGAA, Weinheim.

引用

页码：1401 / 1404

页数：4

共 36 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].