Instability of CHO chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10

被引：32

作者：

Slijepcevic, P ^{[1
]}

Xiao, Y ^{[1
]}

Natarajan, AT ^{[1
]}

Bryant, PE ^{[1
]}

机构：

[1] LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,NL-2300 RA LEIDEN,NETHERLANDS

来源：

CYTOGENETICS AND CELL GENETICS | 1997年 / 76卷 / 1-2期

关键词：

D O I：

10.1159/000134516

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We identified a marker chromosome in the CHO K1 cell line containing amplifications of interstitial telomeric sequences originating from Chinese hamster chromosome 10. Analysis of the progression of this chromosome in two subclones of CHO K1 revealed sensitivity of one amplicon to chromosome breakage, resulting in telomere function at the break site. In addition, two more marker chromosomes, both containing amplifications of interstitial telomeric sequences from chromosome 10, were formed during karyotypic evolution of the CHO K1 subclones. The presence of some of the marker chromosomes was also identified in the radiosensitive xrs 5 cell line derived from CHO K1. These results indicate instability of CHO K1 chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10.

引用

页码：58 / 60

页数：3

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