Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet

被引：45

作者：

Lusic, Hrvoje ^{[2
]}

Gustilo, Estella M. ^{[1
]}

Vendeix, Franck A. P. ^{[1
]}

Kaiser, Rob ^{[3
]}

Delaney, Michael O. ^{[3
]}

Graham, William D. ^{[1
]}

Moye, Virginia A. ^{[1
]}

Cantara, William A. ^{[1
]}

Agris, Paul F. ^{[1
]}

Deiters, Alexander ^{[2
]}

机构：

[1] N Carolina State Univ, Dept Mol & Struct Biochem, Raleigh, NC 27695 USA

[2] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

[3] Dharmacon, Lafayette, CO 80026 USA

来源：

NUCLEIC ACIDS RESEARCH | 2008年 / 36卷 / 20期

关键词：

D O I：

10.1093/nar/gkn703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human mitochondrial methionine transfer RNA (hmtRNA(CAU)(MET)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in hmtRNA(CAU)(MET) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.

引用

页码：6548 / 6557

页数：10

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