Uniform binding of aminoacylated transfer RNAs to the ribosomal A and P sites

被引:86
作者
Fahlman, RP [1 ]
Dale, T [1 ]
Uhlenbeck, OC [1 ]
机构
[1] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA
关键词
D O I
10.1016/j.molcel.2004.10.030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The association and dissociation rate constants of eight different E. coli aminoacyl-tRNAs (aa-tRNAs) for E. coli ribosomes programmed with mRNAs of defined sequences were determined. Identical association and dissociation rate constants were observed for all eight aa-tRNAs in both the ribosomal A and P sites despite substantial differences in tRNA sequence, the type of esterified amino acid, and posttranscriptional modifications. These results indicate that the overall binding of all aa-tRNAs to the ribosome is uniform. However, when either the esterified amino acid or the tRNA modifications were removed, binding was no longer uniform. These results suggest that differences in tRNA sequences and tRNA modifications have evolved to offset differential thermodynamic contributions of the esterified amino acid and the codon-anticodon interaction so that ribosomal binding of all aa-tRNAs remains uniform.
引用
收藏
页码:799 / 805
页数:7
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共 58 条
[1]   Decoding the genome: a modified view [J].
Agris, PF .
NUCLEIC ACIDS RESEARCH, 2004, 32 (01) :223-238
[2]   Single atom modification (O→S) of tRNA confers ribosome binding [J].
Ashraf, SS ;
Sochacka, E ;
Cain, R ;
Guenther, R ;
Malkiewicz, A ;
Agris, PF .
RNA, 1999, 5 (02) :188-194
[3]   THE 3-A CRYSTAL-STRUCTURE OF YEAST INITIATOR TRANSFER-RNA - FUNCTIONAL IMPLICATIONS IN INITIATOR ELONGATOR DISCRIMINATION [J].
BASAVAPPA, R ;
SIGLER, PB .
EMBO JOURNAL, 1991, 10 (10) :3105-3111
[4]   AMINOACYL DERIVATIVES OF NUCLEOSIDES, NUCLEOTIDES AND POLYNUCLEOTIDES .33. STEREOCHEMICAL CONTROL OF RIBOSOMAL PEPTIDYLTRANSFERASE REACTION - ROLE OF AMINO-ACID SIDE-CHAIN ORIENTATION OF ACCEPTOR SUBSTRATE [J].
BHUTA, A ;
QUIGGLE, K ;
OTT, T ;
RINGER, D ;
CHLADEK, S .
BIOCHEMISTRY, 1981, 20 (01) :8-15
[5]   Transfer RNA modification:: influence on translational frameshifting and metabolism [J].
Björk, GR ;
Durand, JMB ;
Hagervall, TG ;
Leipuviene, R ;
Lundgren, HK ;
Nilsson, K ;
Chen, P ;
Qian, Q ;
Urbonavicius, J .
FEBS LETTERS, 1999, 452 (1-2) :47-51
[6]   INFLUENCE OF MODIFICATION NEXT TO THE ANTICODON IN TRANSFER-RNA ON CODON CONTEXT-SENSITIVITY OF TRANSLATIONAL SUPPRESSION AND ACCURACY [J].
BOUADLOUN, F ;
SRICHAIYO, T ;
ISAKSSON, LA ;
BJORK, GR .
JOURNAL OF BACTERIOLOGY, 1986, 166 (03) :1022-1027
[7]   Recognition of acceptor-stem structure of tRNAASp by Escherichia coli aspartyl-tRNA synthetase [J].
Choi, H ;
Gabriel, K ;
Schneider, J ;
Otten, S ;
McClain, WH .
RNA, 2003, 9 (04) :386-393
[8]   RATES OF AMINOACYL-TRANS-RNA SELECTION AT 29 SENSE CODONS INVIVO [J].
CURRAN, JF ;
YARUS, M .
JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (01) :65-77
[9]   Co-variation of tRNA abundance and codon usage in Escherichia coli at different growth rates [J].
Dong, HJ ;
Nilsson, L ;
Kurland, CG .
JOURNAL OF MOLECULAR BIOLOGY, 1996, 260 (05) :649-663
[10]   Contribution of the esterified amino acid to the binding of aminoacylated tRNAs to the ribosomal P- and A-sites [J].
Fahlman, RP ;
Uhlenbeck, OC .
BIOCHEMISTRY, 2004, 43 (23) :7575-7583