Effect of Injection Routes on the Biodistribution, Clearance, and Tumor Uptake of Carbon Dots

被引:314
作者
Huang, Xinglu [1 ]
Zhang, Fan [1 ,2 ]
Zhu, Lei [1 ,2 ]
Choi, Ki Young [1 ]
Guo, Ning [1 ]
Guo, Jinxia [1 ,2 ]
Tackett, Kenneth [3 ,4 ]
Anilkumar, Parambath [3 ,4 ]
Liu, Gang [2 ]
Quan, Qimeng [1 ]
Choi, Hak Soo [5 ]
Niu, Gang [1 ]
Sun, Ya-Ping [3 ,4 ]
Lee, Seulki [1 ]
Chen, Xiaoyuan [1 ]
机构
[1] NIBIB, Lab Mol Imaging & Nanomed LOMIN, NIH, Bethesda, MD 20892 USA
[2] Xiamen Univ, Sch Publ Hlth, Ctr Mol Imaging & Translat Med, Xiamen 361005, Peoples R China
[3] Clemson Univ, Dept Chem, Clemson, SC 29634 USA
[4] Clemson Univ, Lab Emerging Mat & Technol, Clemson, SC 29634 USA
[5] Beth Israel Deaconess Med Ctr, Dept Med, Div Hematol Oncol, Boston, MA 02215 USA
基金
美国国家科学基金会;
关键词
biodistribution; carbon dots; clearance; injection routes; translation tumor uptake; QUANTUM DOTS; IN-VIVO; NANOPARTICLES; TISSUE; TOXICITY; GRAPHENE; BRIGHT; FATE;
D O I
10.1021/nn401911k
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The emergence of photoluminescent carbon-based nanomaterials has shown exciting potential in the development of benign nanoprobes. However, the in vivo kinetic behaviors of these particles that are necessary for clinical translation are poorly understood to date. In this study, fluorescent carbon dots (C-dots) were synthesized and the effect of three injection routes on their fate in vivo was explored by using both near-infrared fluorescence and positron emission tomography imaging techniques. We found that C-dots are efficiently and rapidly excreted from the body after all three injection routes. The clearance rate of C-dots is ranked as intravenous > intramuscular > subcutaneous. The particles had relatively low retention in the reticuloendothelial system and showed high tumor-to-background contrast. Furthermore, different injection routes also resulted in different blood clearance patterns and tumor uptakes of C-dots. These results satisfy the need for clinical translation and should promote efforts to further investigate the possibility of using carbon-based nanoprobes in a clinical setting. More broadly, we provide a testing blueprint for in vivo behavior of nanoplatforms under various injection routes, an important step forward toward safety and efficacy analysis of nanoparticles.
引用
收藏
页码:5684 / 5693
页数:10
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