Measuring Direct Thrombin Inhibitors With Routine and Dedicated Coagulation Assays Which Assay Is Helpful?

被引：65

作者：

Curvers, Joyce ^{[1
]}

van de Kerkhof, Daan ^{[1
]}

Stroobants, An K. ^{[2
]}

van den Dool, Erik-Jan ^{[2
]}

Scharnhorst, Volkher ^{[1
]}

机构：

[1] Catharina Hosp, Clin Lab, NL-5623 EJ Eindhoven, Netherlands

[2] Univ Amsterdam, Acad Med Ctr, Lab Gen Clin Chem, NL-1105 AZ Amsterdam, Netherlands

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2012年 / 138卷 / 04期

关键词：

Coagulation assays; Direct thrombin inhibitors; Monitoring; HEPARIN-INDUCED THROMBOCYTOPENIA; BIVALIRUDIN; DABIGATRAN; ARGATROBAN; MANAGEMENT; THERAPY; WARFARIN; TIME;

D O I：

10.1309/AJCPQOD9WFPEYY0H

中图分类号：

R36 [病理学];

学科分类号：

100103 [病原生物学];

摘要：

The use of direct thrombin inhibitors (DTIs) for prophylactic or therapeutic anticoagulation is increasing because of the predictable bioavailability and short half-life of these DTIs. However, in certain situations, indication of the concentration is warranted. We investigated the effects of 3 DTIs (lepirudin, argatroban, and bivalirudin) in 6 pooled plasma specimens on routine coagulation assays (activated partial thromboplastin time [aPTT], prothrombin time [PT], and thrombin time [TT]) and dedicated DTI assays (Hemoclot, HemosIL, the ecarin clotting time, and a chromogenic ecarin clotting time) on 2 coagulation analyzers. We found routine tests to be nondiscriminative between concentrations of different DTIs in the aPTT. Moreover, for PT and TT, the responses for different DTIs differed. This was similar for ecarin clotting assays. The Hemoclot and HemosIL assays showed identical linear increases for all 3 DTIs. We conclude that dedicated calibrated assays based on a diluted TT (Hemoclot and HemosIL) appear to be the most suitable for monitoring purposes.

引用

页码：551 / 558

页数：8

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