Handheld recirculation system and customized media for microfluidic cell culture

被引：82

作者：

Futai, N

Gu, W

Song, JW

Takayama, S

机构：

[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Macromol Sci & Engn, Ann Arbor, MI 48109 USA

来源：

LAB ON A CHIP | 2006年 / 6卷 / 01期

关键词：

D O I：

10.1039/b510901a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A palm-sized microfluidic recirculation system and customized media enable simplified long-term culture and imaging of cells. The combination of bare Braille display modules, a leveled monolithic surface for complete chip mounting, and a transparent heater improved portability, mechanical stability and optical accessibility. Modification of basal culture media with Leibovitz's L-15 medium enabled an incubator-free culture of carbonate-dependent cells by eliminating the need for exogenous carbon dioxide. This capability is demonstrated through time-lapse recording of proliferation of C2C12 myoblasts and MC3T3-E1 osteoblasts for over 2 weeks in ambient atmosphere without medium exchange. The method opens up new possibilities for portable cell culture and for long-term continuous visual monitoring of cells.

引用

收藏

页码：149 / 154

页数：6

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