Molecular typing of nosocomial strains of Legionella pneumophila by arbitrarily primed PCR

被引：28

作者：

Grattard, F

Berthelot, P

Reyrolle, M

Ros, A

Etienne, J

Pozzetto, B

机构：

[1] CHU ST ETIENNE, COMITE LUTTE CONTRE INFECT NOSOCOMIALES, F-42055 ST ETIENNE, FRANCE

[2] FAC MED ALEXIS CARREL, CTR NATL REFERENCE LEGIONELLOSES, F-69372 LYON 8, FRANCE

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 06期

关键词：

D O I：

10.1128/JCM.34.6.1595-1598.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Arbitrarily primed PCR with two different primers was compared with ribotyping and monoclonal antibody analysis for typing Legionella strains. Applied to 11 epidemiologically unrelated strains, arbitrarily primed PCR resulted in an index of discrimination of 100% with both primers. It was found able to identify an epidemic clone of Legionella pnenmophila serogroup 1 that was isolated from both patients and a hot water circuit of the same hospital.

引用

收藏

页码：1595 / 1598

页数：4

相关论文

共 16 条

[1] RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF RIBOSOMAL-RNA GENES FOR MOLECULAR TYPING OF MEMBERS OF THE FAMILY LEGIONELLACEAE [J].

BANGSBORG, JM ;

GERNERSMIDT, P ;

COLDING, H ;

FIEHN, NE ;

BRUUN, B ;

HOIBY, N .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) :402-406

[2] MOLECULAR FINGERPRINTING OF LEGIONELLA SPECIES BY REPETITIVE ELEMENT PCR [J].

GEORGHIOU, PR ;

DOGGETT, AM ;

KIELHOFNER, MA ;

STOUT, JE ;

WATSON, DA ;

LUPSKI, JR ;

HAMILL, RJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (12) :2989-2994

[3]

GOMEZLUS P, 1993, J CLIN MICROBIOL, V31, P1940, DOI 10.1128/JCM.31.7.1940-1942.1993

[4] ARBITRARILY PRIMED PCR, RIBOTYPING, AND PLASMID PATTERN-ANALYSIS APPLIED TO INVESTIGATION OF A NOSOCOMIAL OUTBREAK DUE TO ENTEROBACTER-CLOACAE IN A NEONATAL INTENSIVE-CARE UNIT [J].

GRATTARD, F ;

POZZETTO, B ;

BERTHELOT, P ;

RAYET, I ;

ROS, A ;

LAURAS, B ;

GAUDIN, OG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) :596-602

[5]

GRATTARD F, 1995, INFECT CONT HOSP EP, V16, P224

[6] DIFFERENTIATION OF PSEUDOMONAS-AERUGINOSA STRAINS BY RIBOTYPING - HIGH DISCRIMINATORY POWER BY USING A SINGLE RESTRICTION-ENDONUCLEASE [J].

GRATTARD, F ;

POZZETTO, B ;

ROS, A ;

GAUDIN, OG .

JOURNAL OF MEDICAL MICROBIOLOGY, 1994, 40 (04) :275-281

[7] PHENOTYPIC VARIATION AMONGST GENOTYPICALLY HOMOGENEOUS LEGIONELLA-PNEUMOPHILA SEROGROUP-1 ISOLATES - IMPLICATIONS FOR THE INVESTIGATION OF OUTBREAKS OF LEGIONNAIRES-DISEASE [J].

HARRISON, TG ;

SAUNDERS, NA ;

HATHTHOTUWA, A ;

HALLAS, G ;

BIRTLES, RJ ;

TAYLOR, AG .

EPIDEMIOLOGY AND INFECTION, 1990, 104 (02) :171-180

[8] NUMERICAL INDEX OF THE DISCRIMINATORY ABILITY OF TYPING SYSTEMS - AN APPLICATION OF SIMPSONS INDEX OF DIVERSITY [J].

HUNTER, PR ;

GASTON, MA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (11) :2465-2466

[9] DEVELOPMENT OF A STANDARDIZED SUBGROUPING SCHEME FOR LEGIONELLA-PNEUMOPHILA SEROGROUP-1 USING MONOCLONAL-ANTIBODIES [J].

JOLY, JR ;

MCKINNEY, RM ;

TOBIN, JO ;

BIBB, WF ;

WATKINS, ID ;

RAMSAY, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 23 (04) :768-771

[10] LEGIONELLA-PNEUMONIA IN TRANSPLANT RECIPIENTS - A CLUSTER OF CASES OF 8 YEARS DURATION [J].

PRODINGER, WM ;

BONATTI, H ;

ALLERBERGER, F ;

WEWALKA, G ;

HARRISON, TG ;

AICHBERGER, C ;

DIERICH, MP ;

MARGREITER, R ;

TIEFENBRUNNER, F .

JOURNAL OF HOSPITAL INFECTION, 1994, 26 (03) :191-202