A comparative proteomic evaluation of culture grown vs nodule isolated Bradyrhizobium japonicum

被引：48

作者：

Sarma, Annamraju D. ^{[1
]}

Emerich, David W. ^{[1
]}

机构：

[1] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA

来源：

PROTEOMICS | 2006年 / 6卷 / 10期

关键词：

bacteroids; Bradyrhizobium japonicum USDA110; metabolic profiling; 2-D PAGE; symbiosis;

D O I：

10.1002/pmic.200500783

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Total protein extract of Bradyrhizobium japonicum cultivated in HM media were resolved by 2-D PAGE using narrow range IPG strips. More than 1200 proteins were detected, of which nearly 500 proteins were analysed by MALDI-TOF and 310 spots were tentatively identified. The present study describes at the proteome level a significant number of metabolic pathways related to important cellular events in free-living B. japonicum. A comparative analysis of proteomes of free-living and nodule residing bacteria revealed major differences and similarities between the two states. Proteins related to fatty acid, nucleic acid and cell surface synthesis were significantly higher in cultured cells. Nitrogen metabolism was more pronounced in bacteroids whereas carbon metabolism was similar in both states. Relative percentage of proteins related to global functions like protein synthesis, maturation & degradation and membrane transporters were similar in both forms, however, different proteins provided these functions in the two states.

引用

页码：3008 / 3028

页数：21

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