Integrated biological and chemical monitoring:: In situ physiological responses of freshwater crayfish to fluctuations in environmental ammonia concentrations
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作者:
Bloxham, MJ
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Univ Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, EnglandUniv Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, England
Bloxham, MJ
[1
]
Worsfold, PJ
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Univ Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, EnglandUniv Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, England
Worsfold, PJ
[1
]
Depledge, MH
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Univ Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, EnglandUniv Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, England
Depledge, MH
[1
]
机构:
[1] Univ Plymouth, Plymouth Environm Res Ctr, Plymouth PL4 8AA, Devon, England
A portable, computer-aided physiological monitoring system (CAPMON) has been integrated with an automated, flow injection (FI) based chemical monitor to enable continuous, long-term recording of cardiac activity in selected aquatic organisms, and total ammonia concentration in the surrounding environment. Heart rate of the freshwater crayfish Pacifastacus leniusculus was recorded using non-invasive infrared emitter/detectors to transduce heart beat from 4 animals simultaneously. Data were collected continuously and stored on a laptop computer. The chemical monitor incorporated a gas diffusion unit and a solid state photometric detector. Remote control and data processing were accomplished using an in-house designed microcomputer. The instrumentation was fully evaluated in the laboratory and the field and was shown to be capable of operating unattended for periods of at least 1 week. An exposure-response experiment showed that 4 h exposures to concentrations of ammonia greater than 5 mg l(-1) had a significant stimulatory effect on heart rate (ANOVA F = 7.6; df = 5; P < 0.0005). The feasibility of using the system in situ was demonstrated in a 2 week field trial in which the integrated monitors were successfully deployed at a landfill leachate lagoon.