Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses

被引：71

作者：

Kim, Won-Il ^{[1
]}

Yoon, Kyoung-Jin ^{[1
]}

机构：

[1] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA

来源：

VIRUS GENES | 2008年 / 37卷 / 03期

基金：

美国农业部;

关键词：

PRRSV; structural protein genes; virus neutralization; gene-swapping;

D O I：

10.1007/s11262-008-0278-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To assess the role of each envelope-associated protein (i.e., ORFs 2-6 products) of type 2 PRRSV in cross neutralization mediated by antibody, chimeric mutants were generated by replacing ORFs of a VR2332-based infectious clone with those of JA142, SDSU73, PRRS124, or 2M11715 that are genetically and antigenically distinct from VR2332 and two-way neutralization assays were performed on those mutants using VR2332, JA142, SDSU73, or PRRS124 antisera. All ORF 5-replaced mutants showed increased susceptibility or resistance against homologous or heterologous antisera, respectively, in comparison to that of the donor strains, but failed to achieve a complete reversion of cross neutralization. In contrast, substitution of ORFs 3-6 completely reversed the susceptibility of the virus to neutralization by antibody. Changes in ORFs 3, 5, and 6 were additively responsible for reversion of the susceptibility, suggesting that the genetic similarity of these ORFs should be considered for better cross neutralization between two different type 2 PRRS viruses.

引用

页码：380 / 391

页数：12

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