Automated solid-phase extraction for determination of amodiaquine, chloroquine and metabolites in capillary blood on sampling paper by liquid chromatography

被引：32

作者：

Lindegårdh, N

Forslund, M

Green, MD

Kaneko, A

Bergqvist, Y ^{[1
]}

机构：

[1] Dalarna Univ Coll, S-78188 Borlange, Sweden

[2] Falun Cent Hosp, Dept Clin Chem, S-79182 Falun, Sweden

[3] Univ Uppsala, Dept Analyt Chem, S-75121 Uppsala, Sweden

[4] Ctr Dis Control & Prevent, Div Parasit Dis, Natl Ctr Infect Dis, Atlanta, GA 30333 USA

[5] Tokyo Womens Med Univ, Sch Med, Dept Int Affairs & Trop Med, Tokyo, Japan

来源：

CHROMATOGRAPHIA | 2002年 / 55卷 / 1-2期

关键词：

column liquid chromatography; ion-pair chromatography; solid-phase extraction; antimalarial drugs; capillary blood;

D O I：

10.1007/BF02492307

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A bioanalytical method using solid-phase extraction and high-performance liquid chromatography (LC) has been developed and validated for the determination of amodiaquine, chloroquine and their metabolites in 100 muL blood applied to sampling paper. The intra-assay precision for all analytes was < 5% at 2000 nM, < 7% at 750 nM and < 10% at 100 nM. The inter-assay precision for all analytes was < 4% at 2000 nM, < 7% at 750 nM and < 12% at 100 nM. The lower limit of quantitation was 100 nM for all analytes. The limit of detection was 30 nM in 100 muL venous blood applied to sampling paper.

引用

页码：5 / 12

页数：8

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