Comparative karyotype of rat and mouse using bidirectional chromosome painting

被引:30
作者
Guilly, MN [1 ]
Fouchet, P [1 ]
de Chamisso, P [1 ]
Schmitz, A [1 ]
Dutrillaux, B [1 ]
机构
[1] CEA, DSV, DRR, F-92265 Fontenay Aux Roses, France
关键词
comparative cytogenetics; heterologous chromosome painting;
D O I
10.1023/A:1009251416856
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A comparative karyotype of rat (Rattus norvegicus) and mouse (Mus musculus) based on chromosome G-banding morphology, heterologous chromosome painting results and available gene mapping data is proposed. Whole chromosome painting probes from both species were generated by PARM-PCR amplification of flow sorted chromosomes. Bidirectional chromosome painting identifies 36 segments of syntenic homology and allows us to propose a nearly complete comparative karyotype of mouse and rat (except for RNO 13 p and RNO 19 p12-13). Seven segments completely covered the RNO chromosomes 3, 5, 8, 11, 12, 15 and 18. Eight segments completely covered the MMU chromosomes 3, 4, 6, 7, 9, 12, 18 and 19. The RNO chromosomes 5, 8, 18 show complete homology with the MMU chromosomes 4, 9 and 18, respectively. Bidirectional hybridization results clearly assign 16 segments to subchromosomal regions in both species. Interpretation of the results allows subchromosomal assignment of all the remaining segments apart from seven distributed on chromosomes MMU 15, MMU 10 B2-D3 and MMU 17 E3-E5. The proposed comparative karyotype shows overall agreement with available comparative mapping data. The proposed repartition of syntenic homologous segments between the two species provides useful data for gene-mapping studies. In addition, these results will enable the reconstruction of the karyotype of the Cricetidae and Muridae common ancestor and the definition of the precise rearrangements which have occurred in both mouse and rat lineages during evolution.
引用
收藏
页码:213 / 221
页数:9
相关论文
共 27 条
[1]   Emerging patterns of comparative genome organization in some mammalian species as revealed by Zoo-FISH [J].
Chowdhary, BP ;
Raudsepp, T ;
Frönicke, L ;
Scherthan, H .
GENOME RESEARCH, 1998, 8 (06) :577-589
[2]  
*DEP GEN, 1998, RATMAP DAT
[3]  
EVANS EP, 1996, GENETIC VARIANTS STR, P1446
[4]   THE RAT AS AN EXPERIMENTAL ANIMAL [J].
GILL, TJ ;
SMITH, GJ ;
WISSLER, RW ;
KUNZ, HW .
SCIENCE, 1989, 245 (4915) :269-276
[5]   Human and porcine correspondence of chromosome segments using bidirectional chromosome painting [J].
Goureau, A ;
Yerle, M ;
Schmitz, A ;
Riquet, J ;
Milan, D ;
Pinton, P ;
Frelat, G ;
Gellin, J .
GENOMICS, 1996, 36 (02) :252-262
[6]   LASER MICRODISSECTION AND SINGLE UNIQUE PRIMER PCR ALLOW GENERATION OF REGIONAL CHROMOSOME DNA CLONES FROM A SINGLE HUMAN-CHROMOSOME [J].
HADANO, S ;
WATANABE, M ;
YOKOI, H ;
KOGI, M ;
KONDO, I ;
TSUCHIYA, H ;
KANAZAWA, I ;
WAKASA, K ;
IKEDA, JE .
GENOMICS, 1991, 11 (02) :364-373
[7]   A GENETIC-LINKAGE MAP OF THE LABORATORY RAT, RATTUS-NORVEGICUS [J].
JACOB, HJ ;
BROWN, DM ;
BUNKER, RK ;
DALY, MJ ;
DZAU, VJ ;
GOODMAN, A ;
KOIKE, G ;
KREN, V ;
KURTZ, T ;
LERNMARK, A ;
LEVAN, G ;
MAO, YP ;
PETTERSSON, A ;
PRAVENEC, M ;
SIMON, JS ;
SZPIRER, C ;
SZPIRER, J ;
TROLLIET, MR ;
WINER, ES ;
LANDER, ES .
NATURE GENETICS, 1995, 9 (01) :63-69
[8]   Why map the rat? [J].
James, MR ;
Lindpaintner, K .
TRENDS IN GENETICS, 1997, 13 (05) :171-173
[9]   Cytogenetic localization of cancer-related genes in the rat and comparative mapping studies in human and mouse [J].
Johansson, Å ;
Helou, K ;
Levan, G .
CYTOGENETICS AND CELL GENETICS, 1998, 81 (3-4) :217-221
[10]   THE GENE MAP OF THE NORWAY RAT (RATTUS-NORVEGICUS) AND COMPARATIVE MAPPING WITH MOUSE AND MAN [J].
LEVAN, G ;
SZPIRER, J ;
SZPIRER, C ;
KLINGA, K ;
HANSON, C ;
ISLAM, MQ .
GENOMICS, 1991, 10 (03) :699-718