Infrared methods for monitoring the protonation state of carboxylic amino acids in the photocycle of bacteriorhodopsin

被引：64

作者：

Dioumaev, AK ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA

来源：

BIOCHEMISTRY-MOSCOW | 2001年 / 66卷 / 11期

关键词：

retinal proteins; FTIR; time-resolved; aspartic acid; glutamic acid; COOH stretch; symmetric and antisymmetric stretching vibrations; carbonyl; carboxyl; carboxylate;

D O I：

10.1023/A:1013135419529

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review deals with the role of carboxylic amino acids in the proton-transport activity of bacteriorhodopsin. The main focus is on the infrared data, which allow direct monitoring of the protonation/deprotonation of specific residues during the proton movement in the course of the photocycle. Additional attention is paid to the potential use of carboxylic acids in proteins as internal sensors, based on the sensitivity of their IR frequencies to the immediate environment.

引用

页码：1269 / 1276

页数：8

共 141 条

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