Soy pinitol acts partly as an insulin sensitizer or insulin mediator in 3T3-L1 preadipocytes

被引:22
作者
Do, Gyeong-Min [2 ]
Choi, Myung-Sook [2 ]
Kim, Hye-Jin [2 ]
Woo, Myung-Nam [2 ]
Lee, Mi-Kyung [3 ]
Jeon, Seon-Min [1 ]
机构
[1] Kyungpook Natl Univ, Inst Genet Engn, Taegu 702701, Kyoungsangbuk D, South Korea
[2] Kyungpook Natl Univ, Dept Food Sci & Nutr, Taegu 702701, Kyoungsangbuk D, South Korea
[3] Sunchon Natl Univ, Dept Food Sci & Nutr, Sunchon 540742, Jeonranam Do, South Korea
关键词
soy pinitol; 3T3-L1; preadipocytes; adipogenesis; oil-red-O staining; real-time PCR; MTT assay;
D O I
10.1007/s12263-007-0071-0
中图分类号
Q3 [遗传学];
学科分类号
071007 [遗传学]; 090102 [作物遗传育种];
摘要
The blood glucose-lowering property of pinitol is mediated via the insulin signaling pathway. This study was carried out to evaluate the effects of soy pinitol on adipogenesis in a 3T3-L1 cell line; 3T3-L1 preadipocytes were treated with pinitol (0-1 mM) together with insulin for 9 days. The regulation of lipid metabolism was assessed by oil-red-O staining of intracellular lipids and real-time PCR of adipogenesis-related factors. The inhibition of cell proliferation was estimated by MTT assay. Pinitol treatment did not inhibit lipid accumulation, nor did it affect expression of adipogenesis-related factors, including ADD1, aP2 and FAS, in a dose-dependent manner. Expression of adiponectin, GLUT4, IRS, C/EBP alpha and PPAR gamma mRNAs, however, increased in cells treated with 0.5 mM and/or 1 mM pinitol. Pinitol treatment did not affect the inhibition of cell growth and proliferation in a dose-dependent manner. Accordingly, we suggest that pinitol is nontoxic to this cell line, and that it enhances adipogenesis by acting as an insulin sensitizer or insulin mediator via the upregulation of adiponectin, GLUT4, IRS, C/EBP alpha and PPAR gamma in 3T3-L1 preadipocytes.
引用
收藏
页码:359 / 364
页数:6
相关论文
共 31 条
[1]
Insulin-like effect of pinitol [J].
Bates, SH ;
Jones, RB ;
Bailey, CJ .
BRITISH JOURNAL OF PHARMACOLOGY, 2000, 130 (08) :1944-1948
[2]
TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN [J].
BIRKENMEIER, EH ;
GWYNN, B ;
HOWARD, S ;
JERRY, J ;
GORDON, JI ;
LANDSCHULZ, WH ;
MCKNIGHT, SL .
GENES & DEVELOPMENT, 1989, 3 (08) :1146-1156
[3]
Pinitol supplementation does not affect insulin-mediated glucose metabolism and muscle insulin receptor content and phosphorylation in older humans [J].
Campbell, WW ;
Haub, MD ;
Fluckey, JD ;
Ostlund, RE ;
Thyfault, JP ;
Morse-Carrithers, H ;
Hulver, MW ;
Birge, ZK .
JOURNAL OF NUTRITION, 2004, 134 (11) :2998-3003
[4]
REGULATED EXPRESSION OF 3 C/EBP ISOFORMS DURING ADIPOSE CONVERSION OF 3T3-L1 CELLS [J].
CAO, ZD ;
UMEK, RM ;
MCKNIGHT, SL .
GENES & DEVELOPMENT, 1991, 5 (09) :1538-1552
[5]
CCAAT ENHANCER BINDING-PROTEIN GENE PROMOTER - BINDING OF NUCLEAR FACTORS DURING DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES [J].
CHRISTY, RJ ;
KAESTNER, KH ;
GEIMAN, DE ;
LANE, MD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) :2593-2597
[6]
Molecular regulation of adipocyte differentiation [J].
Cowherd, RM ;
Lyle, RE ;
McGehee, RE .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 1999, 10 (01) :3-10
[7]
Fat targets for insulin signaling [J].
Czech, MP .
MOLECULAR CELL, 2002, 9 (04) :695-696
[8]
Sterol regulatory element binding protein-1c is a major mediator of insulin action on the hepatic expression of glucokinase and lipogenesis-related genes [J].
Foretz, M ;
Guichard, C ;
Ferré, P ;
Foufelle, F .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12737-12742
[9]
SUBLINES OF MOUSE 3T3 CELLS THAT ACCUMULATE LIPID [J].
GREEN, H ;
KEHINDE, O .
CELL, 1974, 1 (03) :113-116
[10]
Greenwood M., 2001, J Exerc Physiol Online, V4, P41